FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences497387
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20450.4111486629123801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.039.0541041
TATCAAC23300.036.5846862
GGTATCA9300.036.4608271
AACTATA400.00269535334.233862
TCAACGC25350.033.7578124
ATCAACG25300.033.6892283
AACGCAG25200.033.687086
GTGGTAT4100.033.415691
CAACGCA25500.033.2907645
ACGCAGA26600.031.9140767
CAGAGTA25750.031.78712710
AGAGTAC26000.031.4814811
CGCAGAG27500.030.8758248
GCAGAGT28200.029.9970429
TGGTATC4400.029.5656092
GAGTACG29150.025.37732112
AGTACGG29200.025.09929313
TACGGGA8850.023.21873515
TACGGGG13400.023.00214415
TGCCTTA750.002032515822.9193631
Produced by FastQC (version 0.11.5)