FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595595
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT13310.22347400498661002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2450.071.1412370
TAATACA208.45909E-569.08494633
TTAATAC252.557003E-455.23999832
ATTAATA252.5687413E-455.18881231
CTCTTAT252.602053E-455.045071
TAACCAA200.007018624352.37037348
TGCTATA200.007461781651.5657582
CTTATAC2450.047.743171
CGCTAAA305.7956483E-446.79884357
CATTAAT306.346806E-445.94423330
ACCATTA306.370532E-445.90946228
TTATACA2600.044.954762
TATACAC2650.044.1065563
GTATCAA27850.041.382631
ATACACA2900.040.304274
GCTAAAA350.001233234240.14426858
GGTATCA10800.040.137031
TCTTATA350.001371124239.2881972
TACACAT3000.038.9607965
ACTAAGG2950.037.33123427
Produced by FastQC (version 0.11.5)