FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences899315
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39010.43377459510849925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41400.036.9794121
GTGGTAT9000.036.532681
TGGTATC9350.035.52752
GGTATCA18400.034.249391
TATCAAC45650.033.532912
AACGCAG48850.031.5465936
TCAACGC49900.030.8141574
CAACGCA50150.030.5922625
ATCAACG50550.030.5534253
GTACTAG450.004789964330.4439031
AGAGTAC49100.030.22380411
ACGCAGA51600.029.9433527
CAGAGTA50200.029.56152710
CGCAGAG52700.029.3281368
GCAGAGT53350.028.9724249
AGTACGG52750.024.36416213
GAGTACG53250.024.13539112
TATACTG1001.6553686E-523.9719075
ACGGGTA2650.021.98230716
TACGGGG21050.021.81459215
Produced by FastQC (version 0.11.5)