FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences895444
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38440.4292842433474343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34850.040.362541
GTGGTAT6600.035.780351
TATCAAC39900.035.4156572
GGTATCA14500.035.4048421
CACTAGT400.002700786834.2227757
TGGTATC7250.032.5633622
AACGCAG43500.032.3273966
ATCAACG43850.032.1473963
TCAACGC44150.031.8514544
CAACGCA44750.031.5008545
ACGCAGA45700.030.7031447
CAGAGTA44950.030.24087510
AGAGTAC45250.029.96471411
CGCAGAG46950.029.8261368
GCAGAGT47600.029.3485579
AGTACGG45600.025.3045513
GAGTACG46200.024.97591812
GTACGGG49350.022.47974614
TACGGGG18900.021.92083515
TACGGGT8600.021.49952515
Produced by FastQC (version 0.11.5)