FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058552
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT25980.24542960572555717No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23730.22417415488327452No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21630.20433573409714403No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21150.19980123791745708No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19040.17986834846091643No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17350.1639031431616019No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16870.15936864698191494No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16850.15917970964109462No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16290.15388946409812648No Hit
GAGTGAACTCTGTCCCAGATCCACCGCCGCTGAACCTTGATGGGACTCCA16110.15218902803074388No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15410.14557622110203372No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA15380.14529281509080327No Hit
GTCCCAGATCCACCGCCGCTGAACCTTGATGGGACTCCACTTTCTAAAGT15030.1419864116264482No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14380.13584594804978875No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14360.1356570107089685No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13050.12328161488523946No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12980.12262033419236844No Hit
GACCAAGGTAGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT12940.12224245951072787No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG12910.12195905349949743No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12900.12186458482908728No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA12230.11553518391160754No Hit
GAGTATTAATGTCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTTCCTGATCTATAAGGCGT12140.11468496587791624No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11840.1118509057656119No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11740.11090621906151045No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAGTATTAATGTCTGGTTGGCCTGGTATCAGCAGAAA11570.10930025166453797No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11450.10816662761961623No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11300.10674959756346404No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT11130.10514363016649159No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10970.10363213143992926No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGATCCACCGCCG10740.10145935202049593No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10740.10145935202049593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20550.037.1440351
GTGGTAT3900.034.383331
TCGTATG1056.184564E-1032.97513240
TATCAAC23650.032.1101462
TCAACGC24550.030.7930264
CAACGCA25000.030.1013035
ATCAACG25350.029.9568043
AACGCAG26050.029.1518236
AGAGTAC27400.027.59140411
ACGCAGA28050.027.1957667
TACGCTC658.8515173E-426.43746818
CGCAGAG29050.026.2595948
CGTATGC1358.998541E-925.6668741
GCAGAGT29600.025.4233999
AGTACGG22300.024.96386713
GAGTACG22850.024.66376312
CGTCTAA1151.5419446E-624.26635250
ACGGGAT2150.023.97590416
ACGAGAC1602.2955646E-923.63407122
TGTTAGA7450.023.13557670
Produced by FastQC (version 0.11.5)