FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320266
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAACCCCTCCCTCGAGAGTCGAATCAGCATGTCAGTTGACTTGTC59260.448848944076421No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT42770.3239498707078725No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT40010.3030449924484915No Hit
GTTGTAGACAGTGCTCCCACTGGCAGAGATTCGCCCAATCCACTCCAGTC39790.3013786615727437No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC37140.28130694875123646No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG34270.25956890505398156No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32160.24358727710930977No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG31190.23624027279351284No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29790.2256363490387543No Hit
CCATATATCAGGGCGTGAGGGCCTTTCCCTGGTTTCTGCTGATACCAGCC27240.20632205934258702No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG26460.20041415896493586No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT26350.19958099352706196No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26090.19761169340117823No Hit
GAAATAGACGGCCGTATCTGCGGCGGTCACAGAGGTCAGGTTCAGGAAGAACTGGTTCCTGGACAAGTCAACTGA25980.1967785279633044No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25810.19549090865022653No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25610.19397606239954676No Hit
GATATATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATATGGCACAGATTTCA25580.1937488354619448No Hit
GTCTCTGGTGGCTCCATCAACAGTGGGTTTCGCTTCTGGACTTGGTTCCG24100.18253897320691437No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC24090.18246323089438038No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23700.1795092807055548No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT22940.1737528649529716No Hit
GGAGTGGATTGGGCGAATCTCTGCCAGTGGGAGCACTGTCTACAACCCCT22730.1721622763897578No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA22570.17095039938921397No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA22460.1701172339513401No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG22450.17004149163880614No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA21800.1651182413240968No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21520.1629974565731451No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC20870.1580742062584358No Hit
GTCTACAACCCCTCCCTCGAGAGTCGAATCAGCATGTCAGTTGACTTGTCCAGGAACCAGTTCTTCCTGAAC20610.15610490613255207No Hit
GTCCCAGGTGCAACTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACCTGCAGTGT19880.15057571731757086No Hit
GTTCTAGAACATGACACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTC19710.14928809800449305No Hit
GCCCCAGAGATCGAAGTACGAGGGGGGCACCCCCGCCTTCTTTCTCGCAC19160.14512227081512363No Hit
ATATATCAGGGCGTGAGGGCCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAACATCTCTGATGTCCTGACTTGC18790.142319805251366No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18550.14050198975055025No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA18440.13966882431267638No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18280.13845694731213254No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC18220.13800249343692864No Hit
GGACTGGAGTGGATTGGGCGAATCTCTGCCAGTGGGAGCACTGTCTACAA18220.13800249343692864No Hit
GTCCCGGACCTCCTGTTCTAGAACATGACACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGCC17930.13580596637344292No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT17400.1317916238091415No Hit
CACCTGCAGTGTCTCTGGTGGCTCCATCAACAGTGGGTTTCGCTTCTGGA17190.13020103524592772No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC17080.12936786980805384No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG16860.12770153893230607No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATCAGAGATGTTTTAGGCTGGTATCAGCAGAAA16670.1262624349941603No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16660.12618669268162627No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG16650.12611095036909228No Hit
GTATCAACGCAGAGTACGGGACTTTCTGAGAGTCCCGGACCTCCTGTTCTAGAACATGACACATCTGTGGTTCTT16640.1260352080565583No Hit
GTCTCCATCCTCCCTGTTTGCATCTGTAGGAGACAGAGTCACCATCACTT16640.1260352080565583No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16530.12520204261868442No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG16250.12308125786773272No Hit
ATCCACTCCAGTCCCTTCCCGGCGGGCTGCCGGAACCAAGTCCAGAAGCG16210.12277828861759676No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15960.12088473080424703No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC15890.1203545346165091No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT15800.1196728538037032No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT15750.11929414224103325No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15540.11770355367781947No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCATATCCACTGCCG15410.11671890361487762No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15360.11634019205220766No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15350.11626444973967366No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA15290.11580999586446973No Hit
GTCTATTTCTGTGCGAGAAAGAAGGCGGGGGTGCCCCCCTCGTACTTCGA15250.11550702661433379No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA15030.113840695738586No Hit
GTACGGGACTTTCTGAGAGTCCCGGACCTCCTGTTCTAGAACATGACACA15010.11368921111351803No Hit
CTGATATATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCACGGTTCAG14980.11346198417591608No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14860.1125530764255082No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG14850.1124773341129742No Hit
GATGGGCCCTGTCCCAGGTGCAACTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCACAGACCCTGTCCCTCA14850.1124773341129742No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14780.11194713792523628No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14690.11126545711243037No Hit
GTCCAGGAACCAGTTCTTCCTGAACCTGACCTCTGTGACCGCCGCAGATA14670.11111397248736239No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCCGGGTGCCACGGCCCCAGAGATCGAAGTA14610.11065951861215846No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14490.10975061086175059No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC14440.10937189929908064No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG14430.10929615698654666No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14300.10831150692360478No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14270.10808427998600283No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCATCTCCTC14190.1074783414857309No Hit
CTCTGGGGCCGTGGCACCCGGGTCGCCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACC14170.10732685686066293No Hit
CAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGC14170.10732685686066293No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14140.10709962992306095No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC14000.1060392375475851No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACC13990.10596349523505112No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13960.10573626829744914No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13790.10444864898437134No Hit
GGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACCTGCAGTGTCTCTGGTGGCTCCATCAACAGTGGGTTTCG13780.10437290667183734No Hit
CTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCA13430.1017219257331477No Hit
GGTCAGGCTGACCTGGTTCTTGGTCATCTCCTCCCGGGATGGGGGCAGGGTGTACACCTGTGGTTCTCGGGGCTG13370.10126747185794378No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC13330.10096450260780783No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG13240.10028282179500192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)