FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320266
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAACCCCTCCCTCGAGAGTCGAATCAGCATGTCAGTTGACTTGTC50030.3789387896075488No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47800.36204825391246914No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT42720.32357115914520257No Hit
GTTGTAGACAGTGCTCCCACTGGCAGAGATTCGCCCAATCCACTCCAGTC39490.29910639219672397No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC32530.24638974267306737No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30520.23116553785373553No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG29760.22540912210115235No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28860.2185923139730933No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27340.20707948246792693No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27320.20692799784285892No Hit
GTCTACAACCCCTCCCTCGAGAGTCGAATCAGCATGTCAGTTGACTTGTCCAGGAACCAGTTCTTCCTGAAC26560.20117158209027575No Hit
CCATATATCAGGGCGTGAGGGCCTTTCCCTGGTTTCTGCTGATACCAGCC25400.192385473836333No Hit
GAAATAGACGGCCGTATCTGCGGCGGTCACAGAGGTCAGGTTCAGGAAGAACTGGTTCCTGGACAAGTCAACTGA25270.19140082377339113No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25160.19056765833551723No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG25080.18996171983524532No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24420.18496272720800203No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23810.18034244614342868No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23530.17822166139247697No Hit
GATATATGGTGCATCCAGACTGCAAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATATGGCACAGATTTCA23110.17504048426604943No Hit
GGAGTGGATTGGGCGAATCTCTGCCAGTGGGAGCACTGTCTACAACCCCT22990.17413157651564154No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22870.17322266876523368No Hit
GTCTCTGGTGGCTCCATCAACAGTGGGTTTCGCTTCTGGACTTGGTTCCG22760.17238950332735978No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22210.16822367613799039No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC21810.16519398363663082No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC21690.16428507588622293No Hit
GTTCTAGAACATGACACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTC21180.16042221794698946No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT20980.1589073716963097No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT20890.15822569088350377No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA20810.15761975238323186No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA20030.1517118520055807No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA19810.15004552112983294No Hit
GTCCCAGGTGCAACTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCACAGACCCTGTCCCTCACCTGCAGTGT19780.14981829419223094No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT19520.14784899406634724No Hit
ATATATCAGGGCGTGAGGGCCTTTCCCTGGTTTCTGCTGATACCAGCCTAAAACATCTCTGATGTCCTGACTTGC19430.14716731325354135No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19260.14587969394046352No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18600.14088070131322022No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18480.13997179356281234No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT18380.13921437043747245No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG18280.13845694731213254No Hit
GTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTGCCATATCCACTGCCG17200.1302767775584617No Hit
GCCCCAGAGATCGAAGTACGAGGGGGGCACCCCCGCCTTCTTTCTCGCAC17020.12891341593284988No Hit
GTCCCGGACCTCCTGTTCTAGAACATGACACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGCC16920.12815599280751No Hit
GGACTGGAGTGGATTGGGCGAATCTCTGCCAGTGGGAGCACTGTCTACAA16800.12724708505710214No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG16670.1262624349941603No Hit
ATCCACTCCAGTCCCTTCCCGGCGGGCTGCCGGAACCAAGTCCAGAAGCG16600.12573223880642234No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16170.12247531936746078No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC16160.12239957705492681No Hit
GTATCAACGCAGAGTACGGGACTTTCTGAGAGTCCCGGACCTCCTGTTCTAGAACATGACACATCTGTGGTTCTT15920.12058176155411106No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGA15890.1203545346165091No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15850.12005156536637313No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG15720.11906691530343128No Hit
GTACGGGACTTTCTGAGAGTCCCGGACCTCCTGTTCTAGAACATGACACA15690.1188396883658293No Hit
GGGGAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCCGGGTGCCACGGCCCCAGAGATCGAAGTA15680.11876394605329532No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA15210.11520405736419781No Hit
CACCTGCAGTGTCTCTGGTGGCTCCATCAACAGTGGGTTTCGCTTCTGGA15100.11437089192632394No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG14970.11338624186338207No Hit
GTCTCCATCCTCCCTGTTTGCATCTGTAGGAGACAGAGTCACCATCACTT14690.11126545711243037No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA14650.11096248786229441No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14490.10975061086175059No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14440.10937189929908064No Hit
GATGGGCCCTGTCCCAGGTGCAACTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCACAGACCCTGTCCCTCA14440.10937189929908064No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC14340.10861447617374076No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14290.1082357646110708No Hit
GTCTATTTCTGTGCGAGAAAGAAGGCGGGGGTGCCCCCCTCGTACTTCGA14190.1074783414857309No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14150.10717537223559495No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC14140.10709962992306095No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCATCTCCTC14040.10634220679772108No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13910.1053575567347792No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG13830.1047516182345073No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13720.10391845279663342No Hit
GTATCAACGCAGAGTACGGGTAATCGTAATCTTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCTG13580.10285806042115755No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTCTCTTGTCC13530.10247934885848761No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATCAGAGATGTTTTAGGCTGGTATCAGCAGAAA13530.10247934885848761No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13440.1017976680456817No Hit
CTCTGGGGCCGTGGCACCCGGGTCGCCGTCTCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACC13400.10149469879554575No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13380.10134321417047777No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT13340.1010402449203418No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13340.1010402449203418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2750.053.712351
AGTGTCG200.007484114751.5302858
CTAAAGT200.007500947551.5010154
GTATCAA27500.045.468131
TGTATAA1400.044.1437262
GTGTATA1550.042.1075821
GAACCAA13950.041.23873570
TATCAAC32150.038.979512
GGGACTA450.001866974836.89822869
ATCAACG35250.035.5515253
CAACGCA35950.035.0502975
AACGCAG36000.035.0016146
TCAACGC35850.034.956524
AACCTGA19150.033.7958470
CAGAGTA36750.033.28355810
AGAGTAC37000.032.96581311
TGTTAGA5000.032.68638270
AGTAAGA800.00338207232.68638270
GCAGAGT38050.032.6857269
CGCAGAG38300.032.559618
Produced by FastQC (version 0.11.5)