FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131969
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCGTCAGACTGGAGCCCGCTGATGGCCAGGGAGG55470.4900310874237722No Hit
GATTATTACTGTGCGACATGGGATGACAACCTGAATGGTTATGTCTTCGG52090.4601716124734864No Hit
GTGCAGGGTCGTGGGCCCAGTCTGTGCTGTCTCAGCCACCCTCAGCGTCT51920.4586698045617857No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38950.344090695063204No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA36190.31970840190853284No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31520.27845285515769425No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29660.26202130977085064No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28740.25389387871929353No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28210.24921177170046174No Hit
CTCTTGTACTGGAGGCAGCTCCAACATCGGAAGAAATTCTATGACCTGGTACCAGCAGGTCCCAGGAACGGCCCC28080.24806333035622No Hit
GTAATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG25700.22703801959240932No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG24630.21758546391288103No Hit
TAGTAATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC24440.2159069727174507No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23640.20883964136827068No Hit
CCTCACTCACTGTGCAGGGTCGTGGGCCCAGTCTGTGCTGTCTCAGCCAC23300.20583602554486916No Hit
GTCCTGAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23090.2039808510657094No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22610.19974045225620135No Hit
GTGCTGTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCGGAGGGTCAC22350.19744356956771783No Hit
GCCTTGGGCTGACTCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA22010.19443995374431633No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21970.1940865871768573No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21910.19355653732566883No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21120.18657754761835352No Hit
ACTCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGT20660.18251383209257496No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTCAGGACGGTGACCTTGGTC19910.17588820895271867No Hit
GTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCGGAGGGTCACCATCTCTTGTACTGGAGGCAGCTCCAACAT19760.1745630843247474No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT19060.16837916939421485No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18290.16157686297062906No Hit
GTCGTGGGCCCAGTCTGTGCTGTCTCAGCCACCCTCAGCGTCTGGGACCC17620.15565797296569076No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG17450.15415616505399No Hit
TTATTACTATGTATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTA16900.1492973747514287No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16500.1457637090768387No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGGCTCCAGT16120.14240672668597815No Hit
CATTATTACTATGTATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGG15070.13313085429017932No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14650.12942050533185978No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14410.12730030592710578No Hit
AGTCTGTGCTGTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCGGAGG14370.12694693935964677No Hit
CCCCTGAGGGCCGCTCATTATTACTATGTATGAGGAGTTTGGGGGCCGTT13880.122618198908274No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13850.12235317398267974No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG13690.12093970771284372No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA13610.12023297457792571No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGGCTCCAGTCTGACGATG13560.11979126636860198No Hit
GACTCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGG13350.11793609188944221No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12710.11228222681009817No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA12480.11025036904720889No Hit
GGGTAGAGAAGACAGGACTCAGGACATTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA12470.11016202740534414No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12400.1095436359122909No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12340.10901358606110237No Hit
CCCTCAGCGTCTGGGACCCCCGGGCGGAGGGTCACCATCTCTTGTACTGG12310.10874856113550814No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG12040.10636333680515986No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11830.10450816232600009No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT11690.10327137933989357No Hit
ACATGGGATGACAACCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGT11440.10106283829327482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24750.042.507091
GTGGTAT2550.041.796191
TATCAAC26400.039.8275152
ATCAACG27600.038.0958823
TCAACGC27900.037.5630954
GGTATCA9350.037.1385461
CAACGCA28600.036.763865
AACGCAG29350.035.8244066
GCCAGAG20200.035.0312870
TAGACCG400.002648229734.3609925
ACGCAGA32750.032.106657
AGAGTAC32650.031.99590911
CGCAGAG32900.031.9602668
TACTCCG1001.5081241E-830.9248925
CAGAGTA33850.030.86163710
GCAGAGT34300.030.657129
TATACAC1750.029.452283
TCTATAC854.5693905E-628.2972873
TGGTATC3550.028.0695422
AATACCG500.00776540127.57771933
Produced by FastQC (version 0.11.5)