FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1131969
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCGTCAGACTGGAGCCCGCTGATGGCCAGGGAGG53250.47041924292979753No Hit
GATTATTACTGTGCGACATGGGATGACAACCTGAATGGTTATGTCTTCGG48960.43252067856981946No Hit
GTGCAGGGTCGTGGGCCCAGTCTGTGCTGTCTCAGCCACCCTCAGCGTCT48810.4311955539418482No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT36850.32553895027160634No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33740.29806469965166893No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC33610.2969162583074272No Hit
CTCTTGTACTGGAGGCAGCTCCAACATCGGAAGAAATTCTATGACCTGGTACCAGCAGGTCCCAGGAACGGCCCC29560.2611378933522031No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC28330.2502718714028388No Hit
GTAATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG26010.2297766104902166No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG25660.22668465302495033No Hit
TAGTAATAATGAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC24320.21484687301507374No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC23960.21166657390794272No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23290.2057476839030044No Hit
CCTCACTCACTGTGCAGGGTCGTGGGCCCAGTCTGTGCTGTCTCAGCCAC23080.20389250942384465No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG22920.20247904315400864No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG21750.1921430710558328No Hit
GTGCTGTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCGGAGGGTCAC21500.18993453000921404No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC21280.1879910138881895No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21090.18631252269275925No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20940.18498739806478798No Hit
GCCTTGGGCTGACTCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA20680.18269051537630449No Hit
GTCCTGAGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19940.17615323387831291No Hit
GTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCGGAGGGTCACCATCTCTTGTACTGGAGGCAGCTCCAACAT19760.1745630843247474No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACTCAGGACGGTGACCTTGGTC18740.16555223685454284No Hit
ACTCAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCATTCAGGT17990.15892661371468653No Hit
GTCGTGGGCCCAGTCTGTGCTGTCTCAGCCACCCTCAGCGTCTGGGACCC17610.155569631323826No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17550.15503958147263752No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT17490.15450953162144898No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16930.14956239967702295No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG16690.14744220027226895No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC16090.1421417017603839No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15840.13993316071376513No Hit
TTATTACTATGTATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGGTA15620.1379896445927406No Hit
CATTATTACTATGTATGAGGAGTTTGGGGGCCGTTCCTGGGACCTGCTGG15470.13666451996476933No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGGCTCCAGT15080.13321919593204407No Hit
GGGTAGAGAAGACAGGACTCAGGACATTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA14090.12447337338743376No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACATTCTCCAGCATGGCCAG13910.12288322383386824No Hit
AGTCTGTGCTGTCTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCGGAGG13280.11731770039638895No Hit
CCCCTGAGGGCCGCTCATTATTACTATGTATGAGGAGTTTGGGGGCCGTT13270.11722935875452418No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13260.11714101711265944No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA13230.1168759921870652No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13210.11669930890333569No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG12890.11387237636366367No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGGCTCCAGTCTGACGATG12790.11298895994501616No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12740.1125472517356924No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC12640.1116638353170449No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACATTCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA12490.11033871068907364No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA11760.10388977083294684No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC11560.10212293799565184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24200.040.826991
GGTATCA10000.040.8241541
TATCAAC25750.038.09122
TCAACGC27200.035.932934
ATCAACG27550.035.4764373
CAACGCA28150.034.8421025
AACGCAG29250.033.6490446
GCCAGAG18550.032.4430170
GTGGTAT3800.031.5976431
ACGCAGA31450.031.1889277
AGAGTAC31150.031.16863411
CGCAGAG31400.031.137628
CAGAGTA32300.030.16512910
GCAGAGT33600.029.1001329
TGGTATC3900.029.0191862
ACGCTTG1605.365806E-627.71529470
AGTACTT12650.026.30695713
TGTATAA2100.026.129832
GTACTTT12650.026.03575114
GAGTACT13600.024.72159812
Produced by FastQC (version 0.11.5)