FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404268
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9980.24686594041576382No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA200.00752100651.459742
ACATATC306.483812E-445.741993
GTCTAGT350.00138285539.217121
GTATCAA19550.038.790851
TATCAAC21650.034.8611032
GGTATCA8500.034.7186851
AACGCAG23050.033.0454836
ATCAACG22950.033.035883
TCAACGC23100.032.8213654
CAACGCA23500.032.2627035
GTGGTAT3800.031.6059041
AGAGTAC23450.030.74124511
ACGCAGA24750.030.6446467
CGCAGAG24750.030.513538
GGGTATA450.00474229130.5022071
CAGAGTA24100.030.33944110
TGGTATC3850.030.2966442
GCAGAGT25700.029.2520289
GTAGACT605.5972725E-428.5887454
GTATTGA854.6076348E-628.2593961
Produced by FastQC (version 0.11.5)