FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1035601
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC72760.7025871933302498No Hit
GTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCC69120.6674385212065265No Hit
GCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCA66880.6458085691303891No Hit
GATTATTACTGTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGG64150.6194470650375965No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCT55230.5333135058772636No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATGGCCAGGGAGG47270.4564499261781323No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41920.40478910313914335No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG38710.37379260931575No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC38540.37215105045282887No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG35650.3442445497831694No Hit
CCCCAGGGACGGCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCC34530.3334295737451007No Hit
GTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC33940.32773239886790373No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG33900.32734614972368703No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCAC32790.3166277359716725No Hit
GTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA31940.30841994165706677No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30310.29268028903023463No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTC30000.2896868581625549No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29560.2854381175761707No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28330.273560956391506No Hit
ACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGG26770.25849723976705313No Hit
CCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG25600.24719945229871348No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA25050.24188852656573334No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24840.23986071855859542No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA24500.23657760083275314No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22940.2215138842083003No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC22790.22006544991748753No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22460.21687889447769942No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22420.2164926453334827No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGG22220.214561399612399No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22160.2139820258960739No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGT22140.2137889013239655No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT21130.2040361104324928No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20170.19476613097129108No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20130.19437988182707433No Hit
GGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG20090.19399363268285758No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20000.19312457210836992No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT19780.19100020181517785No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19300.186365212084577No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA19250.18588240065430606No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19150.1849167777937642No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18810.1816336600679219No Hit
GCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT18620.1797989766328924No Hit
ATCCAATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGC18460.17825398005602544No Hit
CTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGTCTACTGGTACCAGCAATCCCCAGGGACGGCCCC18180.17555023604650827No Hit
GCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT18060.17439148861385803No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17860.17246024289277434No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGTCCGAAGATG17830.1721705560346118No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17770.17159118231828668No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATC17620.1701427480274739No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17390.16792181544822765No Hit
GACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGG17050.16463869772238537No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC16990.16405932400606027No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16940.1635765125757893No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16750.16174182914075982No Hit
GTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACC16680.16106589313838052No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16460.15894152284518845No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16370.1580724622707008No Hit
ATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC15810.15266497425166642No Hit
GGCCATCAGCGGTCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAA15540.15005779252820342No Hit
GGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTAT15360.14831967137922808No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15140.14619530108603604No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCT14990.14474686679522325No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14770.14262249650203118No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAA14730.14223624735781445No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14680.14175343592754353No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14610.14107749992516422No Hit
CTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC14200.13711844619694263No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13940.13460782675953384No Hit
ATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATG13680.13209720732212504No Hit
ACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGGC13590.13122814674763736No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTC13460.12997283702893295No Hit
GAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATT13390.12929690102655367No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACAC13320.12862096502417436No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13270.12813815359390346No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAG13260.12804159130784926No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC13250.12794502902179508No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA13230.1277519044496867No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGA13210.12755877987757833No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13070.12620690787281974No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC13000.12553097187044043No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12990.12543440958438626No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAG12910.12466191129595278No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12760.12321347700514002No Hit
CAACTAGGCAGGCTGTCATCCAATGTTGCACAGTAATAATCAGCCTCATC12630.1219581672864356No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT12580.12147535585616469No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12490.120606295281677No Hit
CCCCTGAGGGCCGCTGTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGTAGA12390.11964067242113516No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12370.1194475478490268No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12290.11867504956059331No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12100.11684036612556381No Hit
CTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGTCTA12050.11635755469529287No Hit
GAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG11960.11548849412080521No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC11920.11510224497658847No Hit
GCCTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGT11780.11375037297182988No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT11690.11288131239734223No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG11670.11268818782523383No Hit
CTCCAACATCGGAGCTAATTATGTCTACTGGTACCAGCAATCCCCAGGGA11630.11230193868101712No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11540.11143287810652944No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC11280.10892225866912064No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11200.10814976038068716No Hit
GGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC11040.1066047638038202No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC11030.10650820151776601No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACC11030.10650820151776601No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGG11000.10621851465960346No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT10970.1059288278014409No Hit
GTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGG10930.10554257865722416No Hit
GTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCT10880.10505976722695325No Hit
TGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCT10810.10438383122457395No Hit
TCCCTGGGGATTGCTGGTACCAGTAGACATAATTAGCTCCGATGTTGGAGTCGCTTCCAGAACAAGAGATGGTGA10790.10419070665246558No Hit
CCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGTCTACTGGTACCAG10710.10341820836403211No Hit
ATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10700.10332164607797792No Hit
GGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGAC10670.10303195921981537No Hit
AGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGG10610.10245258550349023No Hit
TTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGGCTGCC10570.10206633635927351No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT10560.10196977407321932No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC10500.10139040035689421No Hit
GGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10500.10139040035689421No Hit
GTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGTAGACATAATTAGCT10420.10061790206846073No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTC10400.10042477749635237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2450.047.7103421
GGTATCA7450.038.3020061
GTATCAA15800.038.2961651
AATGTTG30200.036.5896670
TGGTATC3050.036.0563742
TATCAAC17300.034.763532
GCCAGAG18250.034.49422570
CGATTGT800.003004153533.4850970
ATCAACG18600.032.333823
TCAACGC18600.032.332264
ATCGTTT450.004101829631.4247666
CAACGCA19300.031.1595865
GGGAACG1951.5123078E-830.9093170
ATTACGA450.004617328830.67364734
CGCGTGA450.004688949330.5772910
AACGCAG19700.030.5283766
GCGCAAC703.928098E-529.4852411
CGCAGAG20950.028.5649018
AGAGTAC21250.028.49083911
GTCCTAG854.54978E-628.3125381
Produced by FastQC (version 0.11.5)