FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138235
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC34650.30441868331232125No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGG27190.23887861469731644No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25840.22701814651631694No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT25810.22675458055673917No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATGACACTCAGG25360.22280109116307265No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24620.21629979749348774No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAT23420.20575715911037704No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC23140.2032972101543179No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22490.19758661436346625No Hit
GTAATAGACAGCCGTGTCCTCGGCGCTCAGACTGTTCATTTGCAGATACA22230.1953023760471256No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21680.19047033345486653No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20750.18229978870795574No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20490.1800155503916151No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20400.17922485251288178No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG19700.17307498012273387No Hit
GTCTATTACTGTGCAAGGGGGCCAGTTTGGGGGGGTTATCGTTTTGACTA19660.17272355884329685No Hit
ACGTGGGATAGCAGCCTGAGTGTCATGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG19420.1706150311666747No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19100.16780366093117854No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18980.16674939709286746No Hit
CCCAGGCACAGCCCCCAAACTCCTCATTTTTGACAATAGTAAGCGACCCTCGGGGATTCCTGACCGATTCTCTGG18630.16367446089779353No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18420.16182949918074913No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18050.15857885234595667No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17730.1557674821104605No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17720.15567962679060124No Hit
GATTATTACTGCGCAACGTGGGATAGCAGCCTGAGTGTCATGTTATTCGG17450.15330753315440132No Hit
ATCCCACGTTGCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGG16670.1464548182053794No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16590.14575197564650533No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15990.14048065645494998No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15710.13802070749889084No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14990.13169512446902443No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14850.13046514999099482No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14570.12800520103493565No Hit
GCCCCCAAACTCCTCATTTTTGACAATAGTAAGCGACCCTCGGGGATTCCTGACCGATTCTCTGGCTCCAAGT14370.12624809463775055No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14250.1251938307994395No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13990.12290959248309884No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13860.1217674733249285No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13800.12124034140577296No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC13750.1208010648064767No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13720.12053749884689893No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13570.11921966904901009No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT13150.11552974561492135No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13130.11535403497520283No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13110.11517832433548432No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13100.11509046901562506No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12800.11245480941984738No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12750.11201553282055113No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12270.10779847746730685No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12170.10691992426871427No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA12090.10621708170984023No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12040.10577780511054397No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12010.1055142391509662No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATGACACTCAGGC12010.1055142391509662No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11910.10463568595237363No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG11610.10200002635659595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2750.045.0144271
GTATCAA22250.043.1177521
CGTATGC955.456968E-1240.09917441
TCGTATG1009.094947E-1238.0689340
TATCAAC25550.037.7962952
TAATAGA4850.037.555022
AATAGAC4900.037.1718033
TCAACGC26750.035.972294
GTAATAG5200.035.708561
ATCAACG27050.035.7003863
CAACGCA27050.035.5733385
AATAGCG708.1176404E-735.31109262
AACGCAG27450.035.180166
ATAGACA5350.034.687574
CTCGTAT905.0749804E-934.57905639
CTAGCGC1102.910383E-1134.40719628
CAGCCGT5900.032.0350539
AGAGTAC29400.032.02712611
CGCAGAG30400.031.4257748
CAGAGTA30400.030.97360410
Produced by FastQC (version 0.11.5)