FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138235
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33440.2937881896093513No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGG25890.2274574231156132No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGT24750.21744191665165805No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23570.2070749889082659No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23490.20637214634939183No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23140.2032972101543179No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23070.20268222291530308No Hit
GGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC21500.18888893769739992No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG21440.18836180577824438No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21020.18467188234415566No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19980.17553492907879303No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACAT19690.17298712480287462No Hit
GTAATAGACAGCCGTGTCCTCGGCGCTCAGACTGTTCATTTGCAGATACA19390.17035146520709693No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAACATGACACTCAGG19010.16701296305244523No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18710.16437730345666757No Hit
ACGTGGGATAGCAGCCTGAGTGTCATGTTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG18200.1598966821438455No Hit
GTCTATTACTGTGCAAGGGGGCCAGTTTGGGGGGGTTATCGTTTTGACTA18060.15866670766581595No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17520.1539225203934161No Hit
CCCAGGCACAGCCCCCAAACTCCTCATTTTTGACAATAGTAAGCGACCCTCGGGGATTCCTGACCGATTCTCTGG17220.15128686079763845No Hit
GATTATTACTGCGCAACGTGGGATAGCAGCCTGAGTGTCATGTTATTCGG16940.14882691184157928No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16910.14856334588200154No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16360.14373130328974246No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16130.1417106309329796No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG15910.13977781389607594No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCATCTCCTG15890.13960210325635744No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15650.1374935755797353No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15300.13441863938466134No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15250.13397936278536504No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14750.12958659679240228No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14560.1279173457150764No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14270.125369541439158No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT14150.12431527760084693No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCAC13680.12018607756746191No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13610.11957109032844711No Hit
GCCCCCAAACTCCTCATTTTTGACAATAGTAAGCGACCCTCGGGGATTCCTGACCGATTCTCTGGCTCCAAGT13550.11904395840929156No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13500.11860468180999531No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13430.1179896945709805No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13390.11763827329154349No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13340.1171989966922472No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13330.11711114137238794No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13200.11596902221421763No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13010.11429977113689177No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12820.11263052005956589No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC12780.1122790987801289No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12700.11157625622125483No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12110.10639279234955874No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11990.10533852851124768No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11890.10445997531265512No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11870.1042842646729366No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11560.10156074975729969No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11560.10156074975729969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGAC4500.041.1289563
GTATCAA23000.040.697991
TAATAGA4650.039.8022162
GTGGTAT2700.039.3673441
TATCAAC25300.036.8480722
GTAATAG5150.036.6179161
ATAGACA5250.036.5574654
CAGCCGT5350.035.261429
GACTGTT5650.033.46563730
CCGTGTC5650.033.390612
ATCAACG28000.033.294873
TCAACGC28100.032.9309924
CAACGCA28400.032.8244865
CGTGTCC5650.032.78349713
TAGACAG6150.032.3221475
AACGCAG28850.032.1936956
ACTGTTC5900.032.06031831
CGCAGAG30900.029.847848
AGACTGT6350.029.77126729
AGAGTAC30450.029.73901711
Produced by FastQC (version 0.11.5)