FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531403
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19220.36168407028187644No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3900.038.700751
TGGTATC4150.036.3625342
GTATCAA20600.036.1346471
TGTATAG400.00267147434.2964825
GACCGGA1151.6859394E-533.3582770
GGTATCA9700.033.242021
TATCAAC23700.031.6916852
AACGCAG24900.030.1643756
ATCAACG24950.029.8290043
TCAACGC25000.029.7693444
CAACGCA25400.029.3005395
AGAGTAC25400.029.04688511
ACGCAGA26550.028.2897537
CAGAGTA26200.028.15995610
CGCAGAG26800.028.0337688
GCAGAGT27900.027.0565519
GCTAGGG800.001046971125.68369569
AGTACGG27450.024.25236913
GAGTACG27850.024.02725812
ATTCTAA1001.6324277E-524.0120541
Produced by FastQC (version 0.11.5)