FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length23-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTGGGCCATGCTGTGGTCAGCCAGGTGTCCCATGCTCTGGAGCTGC11.1363636363636365No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11.1363636363636365No Hit
GTAAGGCTCAGAGAGAAGCAACCAACTGGAGTTAATTGCCCATTTGGGCTCTTTGTATAATTATGGCAAAGTAGA11.1363636363636365No Hit
AATATACTCAAATTAAAAAATAAATTTAAAAAAATAAAAAGTTAAAATAG11.1363636363636365No Hit
ACCAAAGTTAAATAATATCTCTTGACAAAGCATCCTCATTAGATGAAACA11.1363636363636365No Hit
TGCTGGACCCGACAAGCCCCGCTCTGCTGAAGCGCAAGAATTTCAGGCCGTAAATCTCAATCACGGAACCTC11.1363636363636365No Hit
GAGAGATGCCCATCATACAATTGTTAATGGACAGAGACACCAAACAAGTA11.1363636363636365No Hit
GTCCAGCATTTGCCATGGCCAAGATGACAGGACCCGTATGCTTTAGGATGAAGTTATCATCTACAAATTTCTCCC11.1363636363636365No Hit
ACTCAGATTAAAATTTTTTAAAAATATTAATAAATAATTAAAATCTACAA11.1363636363636365No Hit
ACCAACTACAACCCCTCTCTCAAGAGTCGAGTCACCATCTCAGTAGACATGTCGAAGAATCAGTTCTCCCTGAAG11.1363636363636365No Hit
CAAACTACGAAAACCAAACACGGATAACCGAAATTACCACGCTAGGTAGATCAACTAATCATTGATAAATATGC11.1363636363636365No Hit
CTCTGAGTCCCCTCGGCCTTGCTCTCTGCCCATCCGCCCCTGCTATGGTTTAGAGCCTGGCCCAGCTACTCCAGA11.1363636363636365No Hit
GTCCTTGGCTGGTCGGGCCTCCGGTGTTCTGCTTCTCCCCGCTGAGCTGCTGCCTGGTGAAGAGGCAG11.1363636363636365No Hit
CTTAAACACAAAACCCTAACAACCCGCAGACGATACTGTACAACACGATC11.1363636363636365No Hit
GTGCCAGGAGACGGCGCTCCTTTCAGAACGCTGTTTAAAGACTTTGGACC11.1363636363636365No Hit
GGTATCAACGCAGAGTACGGGTGGCCAAGTTATCATGTCCATCCGCACCA11.1363636363636365No Hit
GAGTACGAGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.1363636363636365No Hit
TAGAATAACAATATATTTTCACCTTTAGTTTTATCAGCAACCCAGGAGTT11.1363636363636365No Hit
AACTACCACTCGCAATAAAAATAAGAAGCCCACAAGGCAGCTGAGAAAACAGGCAAGTATATCCCACACATAAA11.1363636363636365No Hit
CGTCTAACTGTGTAGATTTTGGCATTAGCTGTTTGTATCCTAGCTAGAGGTTTTTCTTGTGCTTCGCGGTCA11.1363636363636365No Hit
AACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCTTCCACCAGGGCC11.1363636363636365No Hit
GAGAAGCGCCAGTCCATGTCCGGAGCGAAGCCGGGGAAGTGACTATCCAG11.1363636363636365No Hit
GTCTTCTGAGGATCCTGCAAGATGGAAGAAGTAGAGAAGAAGAAGAAGAT11.1363636363636365No Hit
CAGGTGGGATGTGGTCATGGTCTTCGCGTTCCGCGACTGGGTCACCTGGC11.1363636363636365No Hit
AAAACTCAAACTACTCGCTCACAGCCCACATTCATGAGAACACTCAGTCAATCCACACCCGCCCAACGTCAAAC11.1363636363636365No Hit
CCTAAATGAGGAAATTACAAGGAAACCTAACCATTCCAGAATTACAAAGCCAGATGGTCAGATAAAAGAAAA11.1363636363636365No Hit
GCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGA11.1363636363636365No Hit
GTCCTAAATACCTCATCACTCTAATCACTCACAAAAGTGAAAACCCTGACATCCCGAAGATTCTTCACCCA11.1363636363636365No Hit
CTCTTCACCACTGTGCTACATACAATCGTGACCTGGCAGCTGTAGCTTCA11.1363636363636365No Hit
GGGGCAAGTGAGAGCCGGACGGACACTGGGCGACTCTGTGCCTCGCTGAG11.1363636363636365No Hit
GCTTATTATGCTGGCAAATGTGGCCTTAACTGGGGAAGTAAATGGCAGCT11.1363636363636365No Hit
CGAGTATAGGGGTAAAAATGGTGGGCCATACGGTACTATATAGTTGGGGCATTCAACTACTCTCACTACAAACC11.1363636363636365No Hit
CTTCATTGCTTCGCCCTCTGGGTAAGAAGCGTGCGGACGATGATGTTCCTGGCGCTCGCACGGGTGGCCAGAGG11.1363636363636365No Hit
GTTCCGGCCGGAAAAAGAGGCCG11.1363636363636365No Hit
CAATGAAAAAAAACCCAAAAAACGGTAAAAAATAATTAAACAACTTCACATACAACAACAATAAATGCACGTT11.1363636363636365No Hit
GATGAGATTGGCATGGCTTTATTTGTTTTTTTTTGTTTTGTTTTGGTTTT11.1363636363636365No Hit
TTAAACATTCAGAGATTCAGTGGTCCACCAAATCCACCATCAAATATTTTCCAAGCCCAAGATGACTCTCAGATA11.1363636363636365No Hit
GGCATGCGTCGCCCGAATCAGTCATCGAAGCAGTCGAGAAGCTCAAAATTCTGGAGAACTGCAGCAGCGAAAGCT11.1363636363636365No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11.1363636363636365No Hit
CTATGACAGCAGCCTGAGTGGTTAAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAA11.1363636363636365No Hit
GCTCACGGGCGCCTCAAGCTCCCTGGCTCAGGCGAGGCCGGCCCGCAGCGGCCTGGGTAGGTGAGACGAGAGGTG11.1363636363636365No Hit
AACTACAGTAAACATTAGAACAACAAGGATTTACAATATATTACACAAACCAAAAACAAAACTACATCCACAAAC11.1363636363636365No Hit
GCAGGAGACTGCGTCATCACTATTTCTCCAGTGTTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGCTGCG11.1363636363636365No Hit
GTACGAGAGAACCAAACCATGAGAACCAGCAGCTCTAGTCAACATAAGAAGCCAGTCATGGATGACCACCGATA11.1363636363636365No Hit
ATACAAAACAGACTTGCTCCGCAAAACACCACTATAACATCCACATG11.1363636363636365No Hit
ACAGGGTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAGGGTCACCAT11.1363636363636365No Hit
CATATGAATATAACTACTAATAAAAAAATCTACAAAAATCTTTTCTACGA11.1363636363636365No Hit
CTCCTTCCCAGCCACTGGCTGCCAGAAACTCATTGAAGTGGACGATGAACGCAAACTTCCTACTTTCTATGAGAA11.1363636363636365No Hit
CCAATATGTTTGTTAGACCAAAATCCTGGAAACAGCCTTCTCCAAAGCAG11.1363636363636365No Hit
AACCTCAACAACCTCTTGTTGCAAACATCCTATTTCATGACGATGGGCACTTCAATAAAGGATCCCCACTCCTT11.1363636363636365No Hit
GTAAATACCTAAATTCCAAAAAAATCTTCATCAAAACTTTAAAAATGCAC11.1363636363636365No Hit
AACAAGAAAACTTATATAATTATTATATTATATAAAATAAGGTAGGGCGC11.1363636363636365No Hit
GATGAACACCTCCACCCCCGTCATAGACTAGACATAATACACCACCAAGACGACCAACAACAACTAGAACAT11.1363636363636365No Hit
CATGAAAACACACAAAGTACAAAAATAATATAATATATTTTAATTTAATGAATTGATGCCAGTCTAAAGAAAAG11.1363636363636365No Hit
ACTTAACTATCTTGGGCTGTGACAAAGTCACATCGTTCACACGGCAGCCA11.1363636363636365No Hit
CAGCAGAATACAAATGTAAAAAATATTATTATTTTTTAGATTCCTGAGGA11.1363636363636365No Hit
CGCGCACGTCGAGCTTGTGCATCAGGCCCAGGCAGGGCTGCCGCGGGCTC11.1363636363636365No Hit
CATAACAACGATCAAAATAAATACAGAATACAGAAAAATAATCCAAAAATCTGTATCTAAAACAAAACTTACGAT11.1363636363636365No Hit
GTATCAACGCAGAGTACGGGAAGGCACATGCAGCGCAAGTAGGTCTACAA11.1363636363636365No Hit
GGTATCAACGCAGAGTAGTTTGTTTTTTTTTTTTTTTTT11.1363636363636365No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11.1363636363636365No Hit
CAGCTCAAAGTACTAAAATAATAAAATTTTTTAAATTTAAAATGGTACGA11.1363636363636365No Hit
ATCCCGCCAAGACCCGCATTCCAACAACATTACATCAAACAGTCACCTCA11.1363636363636365No Hit
GTCTAAGCTGGAATCTGGTCACCTTCCATCCATGCAACAACTTGTTCAAA11.1363636363636365No Hit
CAGTAATACACAGCCGTGTCTTCATCTTTCAGGTTGTTCATTTGCAGATA11.1363636363636365No Hit
ATCAAACACTCCTCAGAAGAACCGTTCTCCACAAAGGTCGCCCAAACCTA11.1363636363636365No Hit
GAACTACACACAAACAAACCATCAAAACAAAACAAAAAAAAGTAA11.1363636363636365No Hit
GACTTTGATTTCTTCTTATTTTTCTTTGACTTTTCATTTTTCTTGTCAATCTCTGGTGGCTGCTTTGCTGTTACA11.1363636363636365No Hit
GAAGACATCCTTATTGACGACAGTTTTGTCCAAGGGGATATCCACAGAGT11.1363636363636365No Hit
ATTAAAAGCAGAACTATTTAATCACATAGTCCACTAAGTACCTACAAATA11.1363636363636365No Hit
TCTCTGCTCTCCATTAAGTGCATTCACTCTGCTTGCCTTGGGCCCAGCCC11.1363636363636365No Hit
TACAACACCTTCCCATACATCAACTACTCCTTCATCACTATCATGACGCTCACCAACATGAACGACCAGAAGA11.1363636363636365No Hit
CAGCTGAATGAAAAATTACCCAGCACAAGACCAAGAGTTTGTGTTAATGA11.1363636363636365No Hit
GCTGCGGCGACAGCTCCAGCAGGAGAAGGAGGAGCTGTTGTACCGGGTCCTTGGGGCCAATGCCTAGACTGCCTT11.1363636363636365No Hit
CAAATCCACGTGCGCCAGGCTCAGGTTGCACATGGACGCCTAGTTGTTGATGATCTGATTGGAGGGTTTGGTGG11.1363636363636365No Hit
CTGTTGCCCAGTGTGGGAGTTGGTTTAAATTCTCCTGACTCCAGTTTATA11.1363636363636365No Hit
CCCGACATCCCCTGCTCCGATAATCCGCGCCACATGGCACTCATCACACTCCTCGACGCGCAACTCGCGTTCGGT11.1363636363636365No Hit
CTCGAACTCCTGACCTCGTGATCTGTCCGTCTCGGTCTTCCAAAGTGCTGGAATTACAGGCATGAGCCACCGCGT11.1363636363636365No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTCTCTTGCGT11.1363636363636365No Hit
GTCTGTGTCTCCAGGGGAGAGAGTCACCCTCTCCTGTAGGGCCAGTCAGA11.1363636363636365No Hit
ACGAGCAGGAGGTGCATGAGCCATCCACCGACACGGCCGCCCACACACTCCCAATTCTAAACCATGCCTGGCCAA11.1363636363636365No Hit
GAGGACTTGTTATTGTTTGTGTTAAGATTGATAAAGAGGGAGCGGCGCGC11.1363636363636365No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11.1363636363636365No Hit
GAATACAAATCATATGCCGTCAGGCTAAGATAAATTCTTTCCACGTACTTCTTCTTCATCTCTTAGGATCTT11.1363636363636365No Hit
CTTATACACATCTGACGCTGCCGACGACTTAATAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAATG11.1363636363636365Illumina Single End PCR Primer 1 (100% over 29bp)
AAATCACAAACCTGAAATCTCAGCACACTCCAAAAATACAAAAACACCATCAACAAAAAAA11.1363636363636365No Hit
GTTTATTAAGAACCTACTGTGTGCCAACCGCTTATACGTCGCTTCTCCCT11.1363636363636365No Hit
GTCTCGGCTCACTGCAATTTCTGTCTCTCGGGTACATGTGATTCTCCTGC11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)