FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656899
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23380.35591468399251636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25300.040.2990571
TCTAGAC350.001384367639.2120363
TATCAAC28300.035.8866352
GGTATCA10450.034.1645741
AACGCAG30150.033.7984356
ATCAACG30700.033.3046883
CAACGCA30950.032.8139535
TCAACGC31150.032.7134174
AGAGTAC30550.031.91045611
CAGAGTA30950.031.49804510
ACGCAGA32150.031.484857
GTGGTAT4700.031.4072251
GCAGAGT32400.030.9336159
CGCAGAG32750.030.7054928
ATACGGT500.00792250427.463061
CTAGACT500.00794313727.4484274
TGGTATC5200.027.0525362
AGTACGG33250.026.63507713
TATAATG658.935875E-426.3927172
GAGTACG34000.026.24945812
Produced by FastQC (version 0.11.5)