FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208331
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATGGCCAGGGAGG85680.7090772313215502No Hit
GCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCA83670.6924427164411077No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC79400.6571047171677298No Hit
GATTATTACTGTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGG63260.5235320454411911No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46100.3815179781036819No Hit
CCCCAGGGACGGCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCC39580.3275592532178683No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC39500.32689718297387055No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37900.3136557780939163No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTC36910.3054626588244446No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG36680.3035592068729512No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33420.2765798444300444No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG33410.27649708564954467No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31910.2640832685745876No Hit
GACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGG31250.25862118906160647No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT31220.2583729127201073No Hit
CCCCTGAGGGCCGCTGTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGTAGA28330.23445562515568996No Hit
ATCCAATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGC28150.23296596710669512No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27460.22725561125221486No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27340.22626250588621827No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27230.22535215930072142No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA26890.22253836076373112No Hit
ACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGG26890.22253836076373112No Hit
CCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG26030.2154211056407557No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25700.21269006588426514No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC25610.2119452368597677No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25340.20971074978627544No Hit
GTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGG24990.20681419246878543No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24920.20623488100528745No Hit
TCCCTGGGGATTGCTGGTACCAGTAGACATAATTAGCTCCGATGTTGGAGTCGCTTCCAGAACAAGAGATGGTGA24140.19977969612630977No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGT23720.19630382734532179No Hit
GTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGTAGACATAATTAGCT23700.1961383097843223No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATC22900.18951760734434522No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC22860.18918657222234636No Hit
GGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG22710.18794519051485065No Hit
GTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA22670.18761415539285178No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22560.18670380880735493No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22410.18546242709985922No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22340.18488311563636123No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22170.1834762163678661No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT21870.18099345295287467No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21850.18082793539187525No Hit
CCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGT21570.17851068953788327No Hit
CTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCA21430.17735206661088726No Hit
ATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATG21320.17644172002539038No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20260.1676692892924207No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20120.1665106663654247No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGG19930.16493824953593014No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19900.164689973194431No Hit
ACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGGC19770.16361410904793472No Hit
GCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT19450.16096582807194387No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC19410.160634792949945No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGTCCGAAGATG19000.15724168294945673No Hit
GCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT18990.15715892416895702No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18780.15542098977846303No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18580.15376581416846874No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT18420.15244167368047332No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG18310.15153132709497646No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAA18230.15086925685097874No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18140.15012442782648133No Hit
GTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACC17990.14888304611898562No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17980.1488002873384859No Hit
CAACTAGGCAGGCTGTCATCCAATGTTGCACAGTAATAATCAGCCTCATC17720.14664855904549332No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAG17650.14606924758199533No Hit
CTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGTCTACTGGTACCAGCAATCCCCAGGGACGGCCCC17510.14491062465499932No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTGTTCA16920.1400278566055162No Hit
ATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC16880.13969682148351734No Hit
CTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC16190.1339864656290371No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16110.13332439538503937No Hit
GGCCATCAGCGGTCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAA16110.13332439538503937No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16050.13282784270204107No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15890.13150370221404567No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTC15680.12976576782355165No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCT15510.12835886855505652No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15100.12496575855456823No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC14900.12331058294457398No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA14800.12248299513957682No Hit
GGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC14780.1223174775785774No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14530.12024850806608454No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14330.11859333245609026No Hit
GTCAGGGACCCCTGAGGGCCGCTGTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGGTA14200.11751746830959398No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13930.1152829812361017No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG13920.11520022245560199No Hit
GTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCC13910.11511746367510226No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC13810.11428987587010513No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13800.11420711708960542No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC13790.11412435830910571No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG13750.11379332318710685No Hit
GTCATCCAATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGAC13730.11362780562610743No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT13660.11304849416260941No Hit
GGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTAT13320.11023469562561913No Hit
GAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG13270.10982090172312058No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13160.10891055513762371No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG12730.10535192757613601No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT12720.1052691687956363No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACAC12680.10493813367363743No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12610.10435882221013944No Hit
GGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGA12590.10419330464914No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12520.103613993185642No Hit
GAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATT12410.10270364660014517No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT12390.10253812903914573No Hit
TCTTCGGACCGGAGACCGCTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC12380.10245537025864603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC200.007422650751.63760832
GTATCAA18950.040.769191
GGTATCA7400.039.904841
TATCAAC20450.037.4305842
GCCAGAG35200.036.53708370
TCAACGC21850.035.0322844
ATCAACG22400.034.1721153
CAACGCA22250.034.0939455
AACGCAG22800.033.4220546
AATGTTG39900.033.17662470
GTGGTAT2700.033.064991
TCGTATG958.538336E-932.7846640
TGGTATC2800.030.6476382
CAGAGTA24750.030.2340510
AGAGTAC24850.029.97425311
ACGCAGA25450.029.941967
CGCAGAG25600.029.766528
GCAGAGT25950.029.2327679
AGGGGGG1952.856359E-828.95546570
ACTACGC1551.8189894E-1228.82240127
Produced by FastQC (version 0.11.5)