FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108801
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC113381.0225459753373238No Hit
ACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCCCTCA72040.6497108137528737No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT55610.5015327367128998No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGTCAGGGAGG54490.4914317357217391No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC52280.47150029626596657No Hit
CTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCC52070.46960635858012395No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC49090.4427304809429285No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA48660.43885241806239356No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC43780.3948409137437646No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43570.392946976057922No Hit
TTCCTATAGAGGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCA42860.38654366292959697No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC40710.36715334852692233No Hit
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTC40650.36661222347382444No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG40400.3643575357525832No Hit
GACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCCCTC39600.35714253504461124No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC36970.3334232202171535No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG36040.3250357818941361No Hit
GCCTTGGGCTGACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATA34140.30790015521270275No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG33390.301136092048979No Hit
GTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA31410.2832789652967485No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31340.2826476527348009No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC30590.2758835895710772No Hit
CCCAGGGACGGCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG29760.2683980263365563No Hit
ATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT29110.26253583826132915No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG27770.25045071207547615No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26690.240710461119714No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25700.23178189774359872No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCC25130.22664120973916868No Hit
CCCCTGAGGGCCGCCGATCATTCCTATAGAGGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGATA23770.2143757085356164No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGACCATCAGTGGGCTCCGGT23490.21185045828782623No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC23010.20752145786304305No Hit
CTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23000.2074312703541934No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGG22530.2031924574382599No Hit
GGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTCCTAGGTCAGCCCAAGGCCAA21310.19218958135860267No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA21100.19029564367276003No Hit
GTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTCCTAGGTCAGCCCAAGGCCAACCCCA20740.18704889335417266No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20360.183621768017886No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20040.1807357677346972No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT19860.1791123925754035No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGC19790.17848108001345597No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA19500.17586564225681614No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG19070.17198757937628123No Hit
CTGTCATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAG19050.17180720435858193No Hit
GTGCAGCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTCCTAGGTCAGC19050.17180720435858193No Hit
ATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATG19030.17162682934088264No Hit
GTCCCTGGGAGGTGCTGGTACCAGGATACAGAAATCTTTCCGACGTTGGA19010.17144645432318334No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19000.17135626681433366No Hit
TCCCAGGGACGGCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCC18440.16630576631875332No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG17820.16071414077007506No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17640.1590907656107814No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTG17580.15854964055768347No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT17460.1574673904514877No Hit
GTCATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCC17380.15674589038069048No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17280.155844015292194No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC17100.15422064013290032No Hit
CCCTCAGACTGTCATCCCATGCTGCACAGTAATAATCACCCTCATCCTCG16730.1508837023054633No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16700.15061313977891433No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATATCTTGTTCTGG16670.15034257725236538No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC16330.1472762019514773No Hit
GCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAA15910.14348832657979205No Hit
CCATATCTTGTTCTGGAAGCAGCTCCAACGTCGGAAAGATTTCTGTATCCTGGTACCAGCACCTCCCAGGGACGG15830.14276682650899486No Hit
CCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15700.1415943888939494No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15110.13627332587182012No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC14950.13483032573022571No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTG14870.13410882565942853No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14850.13392845064172923No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATATCTTGTTCTGGAAGCAG14790.1333873255886313No Hit
ATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCAC14620.1318541379381873No Hit
CTGATGGTCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAATCGGTC14430.13014057527004397No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14400.129870012743495No Hit
ATCTTGTTCTGGAAGCAGCTCCAACGTCGGAAAGATTTCTGTATCCTGGTACCAGCACCTCCCAGGGACGGCCCC14290.12887795014614886No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG14290.12887795014614886No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT14250.12851720011075027No Hit
TCCCTGGGAGGTGCTGGTACCAGGATACAGAAATCTTTCCGACGTTGGAGCTGCTTCCAGAACAAGATATGGTGA14160.1277055125311034No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG14110.12725457498685516No Hit
GCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGTCAGGGAGGCTGAGGTGCCAGACGTGGAG13970.12599194986296008No Hit
GGGTCACCATATCTTGTTCTGGAAGCAGCTCCAACGTCGGAAAGATTTCT13890.1252704497921629No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC13800.12445876221251603No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13740.12391763715941814No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG13630.122925574562072No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG13590.1225648245266734No Hit
TCCTCGGACCGGAGCCCACTGATGGTCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAATCGGTCAGGGACC13520.12193351196472586No Hit
GACCATCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGGGGGC13510.1218433244558762No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATATCTTGTTCTGGAAGCAGCTCCAACGTCGGAAAGATTTCTGTATC13490.12166294943817692No Hit
GTACCAGCACCTCCCAGGGACGGCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGA13340.12031013680543216No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13270.11967882424348464No Hit
GTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGATACAGAAATCTTT13200.11904751168153709No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13200.11904751168153709No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGG12830.11571057385410005No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA12820.11562038634525042No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12780.11525963630985181No Hit
GTGCCAGACGTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCCG12710.11462832374790426No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG12650.11408719869480638No Hit
TCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA12630.11390682367710707No Hit
GTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT12590.11354607364170846No Hit
GCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGT12550.11318532360630987No Hit
GTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGGGATGACAGTCTGA12500.11273438606206163No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG12480.11255401104436234No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC12460.11237363602666303No Hit
TGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGT12450.11228344851781338No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG12250.1104796983408204No Hit
TTACTGTGCAGCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAA12220.11020913581427147No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12200.11002876079657216No Hit
CACTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGC12090.109036698199226No Hit
CCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACA11970.10795444809303023No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC11940.10768388556648127No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT11900.10732313553108268No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11890.10723294802223303No Hit
GTGCTGGTACCAGGATACAGAAATCTTTCCGACGTTGGAGCTGCTTCCAG11800.10642126044258617No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAG11720.10569976037178898No Hit
CCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTC11510.10380582268594635No Hit
GACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGG11400.10281376008860021No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACTG11370.10254319756205126No Hit
GTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGAC11290.10182169749125407No Hit
GCTGTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACT11270.10164132247355477No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA11090.10001794731426107No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATATCG200.007500805451.500718
GGTATCA7200.047.2388081
AGGGGGG3150.045.89935370
GTATCAA20300.043.49441
TCTCGTA1950.042.53897538
TATGCCG2300.040.71803343
TATACAC2700.039.4202963
TCGTATG2200.039.32245340
ATCTCGT2200.039.2564437
AACGTAG350.001379559739.24217618
TATCAAC22450.039.1512452
CGTATGC2300.039.151141
AGCGTCA1009.094947E-1237.7671853
TATCTCG2300.037.524136
TTATACA3050.037.1480522
ATCAACG23550.037.0309453
CTTATAC2900.036.724841
CAACGCA23900.036.3449945
TCAACGC24000.036.3366134
CACGAGA2850.036.14736621
Produced by FastQC (version 0.11.5)