FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108801
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC103290.9315467789080276No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC60380.5445521784341825No Hit
CTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCC56200.5068537997350291No Hit
ACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCCCTCA53900.4861106726996097No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC50810.45824273246506814No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49950.45048660670399826No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC47440.42784954198273634No Hit
TTCCTATAGAGGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCA41380.37319591161984883No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA40850.3684159736508174No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC40720.367243536035772No Hit
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTC39220.3537154097083246No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38740.3493864092835414No Hit
GTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA36250.32692971957997874No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35900.323773156770241No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35440.31962453136315716No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG34720.3131310307259824No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG34070.3072688426507552No Hit
ATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT31720.2860747780710876No Hit
CCCAGGGACGGCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG31340.2826476527348009No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30780.2775971522392206No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29770.268488213845406No Hit
GCCTTGGGCTGACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATA28690.25874796288964386No Hit
GACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCCCTC28440.25649327516840265No Hit
CTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC26400.23809502336307417No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGTCAGGGAGG26240.23665202322147977No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGG25510.23006833507545538No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG24990.22537858461527363No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24330.21942620903119678No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT24300.21915564650464783No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24060.21699114629225624No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGACCATCAGTGGGCTCCGGT23050.20788220789844164No Hit
CCCCTGAGGGCCGCCGATCATTCCTATAGAGGAGGAGTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGATA22910.20661958277454656No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21630.19507558164179145No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGC21440.1933620189736481No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACTGTGACCTTGGTCCCAGTTCCGAAGACATAAGGCCCC21270.19182883132320408No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20740.18704889335417266No Hit
GGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTCCTAGGTCAGCCCAAGGCCAA20570.18551570570372863No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20540.18524514317717966No Hit
GTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTCCTAGGTCAGCCCAAGGCCAACCCCA20430.18425308057983353No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19780.17839089250460632No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG19670.17739882990726016No Hit
TCCCAGGGACGGCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCC19090.17216795439398053No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTG19040.17171701684973228No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATATCTTGTTCTGG18880.17027401670813788No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC18690.16856045403999456No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18240.16450201614176033No Hit
ATCTTGTTCTGGAAGCAGCTCCAACGTCGGAAAGATTTCTGTATCCTGGTACCAGCACCTCCCAGGGACGGCCCC18170.1638707035798128No Hit
GTCCCTGGGAGGTGCTGGTACCAGGATACAGAAATCTTTCCGACGTTGGA18170.1638707035798128No Hit
CCATATCTTGTTCTGGAAGCAGCTCCAACGTCGGAAAGATTTCTGTATCCTGGTACCAGCACCTCCCAGGGACGG18000.16233751592936874No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA17980.16215714091166947No Hit
CCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17980.16215714091166947No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG17880.16125526582317296No Hit
GTGCAGCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAAGGTCACAGTCCTAGGTCAGC17780.16035339073467647No Hit
GGGTCACCATATCTTGTTCTGGAAGCAGCTCCAACGTCGGAAAGATTTCT17690.15954170315502963No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATATCTTGTTCTGGAAGCAG16830.1517855773939598No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16480.14862901458422206No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG16400.14790751451342488No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTG16400.14790751451342488No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16220.14628413935413118No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15940.143758889106341No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG15930.14366870159749134No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15900.1433981390709424No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15680.14141401387625013No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15560.14033176377005432No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT15560.14033176377005432No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT15410.13897895113730957No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATATCTTGTTCTGGAAGCAGCTCCAACGTCGGAAAGATTTCTGTATC15240.13744576348686555No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15140.13654388839836906No Hit
GCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAACTGGGACCAA14690.13248545050013483No Hit
TCCCTGGGAGGTGCTGGTACCAGGATACAGAAATCTTTCCGACGTTGGAGCTGCTTCCAGAACAAGATATGGTGA14660.13221488797358588No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14460.13041113779659289No Hit
GTACCAGCACCTCCCAGGGACGGCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGA14230.12833682509305097No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG14190.12797607505765238No Hit
GGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG14160.1277055125311034No Hit
CTGATGGTCAGGGAGGCTGAGGTGCCAGACGTGGAGCCAGAGAATCGGTC14130.12743495000455446No Hit
TCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA14120.1273447624957048No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC13980.12608213737180973No Hit
GCCCCCAAACTCCTCCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGT13720.12373726214171886No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13560.12229426200012447No Hit
GACCATCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGGGGGC13510.1218433244558762No Hit
GTTTGGGGGCCGTCCCTGGGAGGTGCTGGTACCAGGATACAGAAATCTTT13450.12130219940277831No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC13330.12021994929658252No Hit
GTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT13300.11994938677003357No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13220.1192278866992364No Hit
CTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC13190.11895732417268744No Hit
CTGTCATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAG13140.1185063866284392No Hit
CCTCTATAGGAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTC12890.11625169890719797No Hit
GGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC12600.11363626115055814No Hit
GCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA12600.11363626115055814No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12570.11336569862400918No Hit
CACTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGC12400.11183251097356514No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG12360.11147176093816655No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT12350.1113815734293169No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12350.1113815734293169No Hit
GTGCCAGACGTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCCG12300.11093063588506864No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACTG12290.110840448376219No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAG12190.10993857328772251No Hit
GCTGTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACT12090.109036698199226No Hit
TTACTGTGCAGCATGGGATGACAGTCTGAGGGGGCCTTATGTCTTCGGAA12050.1086759481638274No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG11960.10786426058418057No Hit
GTGCTGGTACCAGGATACAGAAATCTTTCCGACGTTGGAGCTGCTTCCAG11840.10678201047798477No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA11710.10560957286293933No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG11680.1053390103363904No Hit
GTCATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCC11660.10515863531869109No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG11620.1047978852832925No Hit
ATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATG11580.10443713524789389No Hit
GTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGGGATGACAGTCTGA11580.10443713524789389No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11560.1042567602301946No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGGAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC11540.10407638521249529No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTATGCAGGGTCCTGGGCCCAGT11490.10362544766824705No Hit
TCAGTGGGCTCCGGTCCGAGGATGAGGGTGATTATTACTGTGCAGCATGG11400.10281376008860021No Hit
GTCCCTGACCGATTCTCTGGCTCCACGTCTGGCACCTCAGCCTCCCTGAC11390.10272357257975057No Hit
CTCACTATGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCA11170.10073944738505827No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC11160.10064925987620862No Hit
ACCATATCTTGTTCTGGAAGCAGCTCCAACGTCGGAAAGATTTCTGTATC11150.10055907236735898No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGG11130.10037869734965967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8400.043.2318531
GTATCAA21450.041.5263061
GTGGTAT2750.041.1110461
TGGTATC2800.039.1357232
TATCAAC23300.038.3588832
TACGGGT853.1795935E-936.2744515
ATCAACG24600.035.914183
CAACGCA24900.035.206435
TCAACGC25000.035.0656054
AACGCAG25250.034.4471866
GCCTAAT951.9013388E-434.3720970
ACAGTAA24650.033.11712670
CGCAGAG27500.031.3924738
AGAGTAC27350.031.31695611
AACAAGG44850.031.30657670
CAGAGTA27700.031.0449410
ACGCAGA28100.030.9590157
GTGTTAC450.00478373130.4526271
GCAGAGT29100.029.7854929
AGTACGG23350.028.61176113
Produced by FastQC (version 0.11.5)