FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48991
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT12592.5698597701618664No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC1640.334755363229981No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT1110.22657222755199935RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT780.1592129166581617RNA PCR Primer, Index 39 (96% over 25bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG730.1490069604621257No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG490.10001837072115287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)