FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48991
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20774.239554203833357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACCT150.003195371563.798775
TCCAGAA200.004481978758.55531359
GGTATCA1650.040.599221
TACGGGA608.3552144E-426.6372415
TACGGGG1009.368723E-725.5979516
GTATCAA2800.023.924542
GTACTTT700.002063924422.80857514
AGTACTT700.002063924422.80857513
AGAGTAC2850.022.40842412
CAGAGTA2750.022.06211310
AACGCAG2900.021.9995776
TACGGGT750.003044618821.33162516
GAGTACT750.003081078621.28800212
CGCAGAG3000.021.2880028
TATCAAC3300.021.2662583
CAACGCA3000.021.2662585
ATCAACG3200.020.9339734
GCAGAGT2900.020.9209699
ACGCAGA3150.020.2742888
ACTTTTT800.004425355219.998416
Produced by FastQC (version 0.11.5)