FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009117
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT59030.5849668571632427No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37560.372206592496212No Hit
GAGTTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA36550.36219784227200613No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCACT35160.34842342364661383No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34800.3448559483191741No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT34270.3396038318648878No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34130.33821648034866125No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33330.33028875739879515No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32730.32434296518639566No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGC32700.3240456755757756No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32590.32295561367016906No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC32000.31710891799464286No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTTGGCCGAACGTC29350.2908483357232115No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG27350.2710290283485463No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26670.2642904638411602No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26390.26151576080870703No Hit
GTATGTTAACTCTTCTCCGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT25970.25735370626002735No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25140.24912869369954127No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT23950.23733620581161552No Hit
CCTTATAGATGAGGAGCTTAGGGACTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAACTATTAATCGGCTGA23940.23723710927474215No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCCGATTAATAGTTGGTTGGCCTGGTATCAGCAGAAG23740.23525517853727565No Hit
CATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTG23220.23010215861986272No Hit
GAGTGAACTCTGTCCCAGATCCAGTGCCGCTGAACCTTGATGAGACCCCA22680.22475094562870312No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22330.2212825668381367No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21600.21404851964638394No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21080.20889549972897098No Hit
GTCCCTAAGCTCCTCATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCACTGGAT20850.2066162793808845No Hit
GTTAACATACTGGTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAACT20800.20612079669651784No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20650.20463434864341798No Hit
GGTATCAGCAGAAGCCAGGGAAAGTCCCTAAGCTCCTCATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCTCAT19970.19789578413603182No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19610.19432830880859206No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19540.1936346330504788No Hit
GTCCCAGATCCAGTGCCGCTGAACCTTGATGAGACCCCACTTTCTAAATT19360.19185089538675892No Hit
ATCTGGGACAGAGTTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATT19310.19135541270239229No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19250.19076083348115233No Hit
GAGTTAACATACTGGTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAACT18710.18540962048999274No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18520.18352678628939953No Hit
ATGTTAACTCTTCTCCGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAA18450.18283311053128626No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18120.1795629248144665No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17760.17599544948702678No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17690.17530177372891348No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT17570.1741126152864336No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17310.17153610532772712No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17030.16876140229527398No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16890.16737405077904743No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16570.164202961599101No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16540.16390567198848102No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16530.1638065754516077No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC16510.16360838237786104No Hit
CATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCA16490.16341018930411438No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC16210.16063548627166127No Hit
ACCTTGATGAGACCCCACTTTCTAAATTAGACGCCTTATAGATGAGGAGCTTAGGGACTTTCCCTGGCTTCTG16110.15964452090292802No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15940.15795987977608147No Hit
GCTTAGGGACTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAACTATTA15310.15171679795306195No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15020.1488429983837355No Hit
TCTCCACCCTGGTCCCTTGGCCGAACGTCGGAGAAGAGTTAACATACTGGTGGCAGTAATAAGTTGCAAAATCAT14640.1450773299825491No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14470.14339268885570255No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14170.14041979274950278No Hit
GTATCAGCAGAAGCCAGGGAAAGTCCCTAAGCTCCTCATCTATAAGGCGT13990.13863605508578292No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13940.13814057240141628No Hit
GGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC13840.13714960703268303No Hit
CCCTGGTCCCTTGGCCGAACGTCGGAGAAGAGTTAACATACTGGTGGCAGTAATAAGTTGCAAAATCATCAGG13700.13576225551645646No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13480.1335821317052433No Hit
GGATCTGGGACAGAGTTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGA13130.1301137529146769No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12940.1282309187140837No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC12840.12723995334535046No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12630.12515892607101062No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12290.12178964381731752No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12130.1202040992273443No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12110.12000590615359764No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12080.11970861654297767No Hit
GCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCACCAGTATGTTAACTCTTCTCCGACGTTCGGCCAAGGGA11950.11842036156362444No Hit
GCACTGGATCTGGGACAGAGTTCACTCTCACCGTCAGCAGCCTGCAGCCT11910.11802397541613113No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11890.11782578234238447No Hit
CTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGG11650.11544746545742465No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT11480.11376282433057812No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11460.11356463125683147No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11440.11336643818308481No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11430.11326734164621148No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11330.11227637627747823No Hit
ACTTATTACTGCCACCAGTATGTTAACTCTTCTCCGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAA11110.11009625246626506No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTCGGAGA11070.10969986631877175No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10430.1033576879588789No Hit
GCTGATACCAGGCCAACCAACTATTAATCGGCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATG10430.1033576879588789No Hit
CCAGTATGTTAACTCTTCTCCGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT10350.1025649156638923No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10270.1017721433689057No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10160.10068208146329911No Hit
GATTAATAGTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGTCCCTA10150.10058298492642578No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10110.10018659877893248No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10100.10008750224205915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1850.044.657031
GTATCAA18150.040.5870481
GGCTATA451.01471946E-438.2479171
TATCAAC20400.036.2577062
ATCAACG22200.033.472863
CAACGCA22500.032.8736535
TCAACGC22600.032.7281954
AACGCAG23700.031.2091666
AGAGTAC25050.028.97789211
CAGAGTA25400.028.84947410
GCCCATA605.492962E-428.6859441
CGCAGAG26000.028.4483538
TGTTAGA8100.028.23179670
ACGCAGA26300.028.1238487
GCAGAGT26950.027.573199
GTGTAAG1359.649739E-925.4986131
GTACTAT700.001351180724.587951
AGTACGG21300.023.90419613
GAGTACG21400.023.79249412
GTATTGC902.1584661E-422.9487511
Produced by FastQC (version 0.11.5)