FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009117
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53530.5304637618829134No Hit
GAGTTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA36380.36051320114515956No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35420.3509999336053203No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCACT33970.336630935758688No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33060.3276131509032154No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31680.3139378288146964No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31660.31373963574094976No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31630.3134423461303298No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGC30540.30264082361113726No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC30000.29728961061997766No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29290.2902537565019715No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTTGGCCGAACGTC27270.2702362560535597No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC27260.2701371595166864No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG25280.2505160452157678No Hit
GTATGTTAACTCTTCTCCGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCAT25080.24853411447830132No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24390.24169645343404184No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24080.23862446079096875No Hit
GAGTGAACTCTGTCCCAGATCCAGTGCCGCTGAACCTTGATGAGACCCCA23920.23703891620099554No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23560.23347144087355579No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCCGATTAATAGTTGGTTGGCCTGGTATCAGCAGAAG23410.2319849928204559No Hit
CCTTATAGATGAGGAGCTTAGGGACTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAACTATTAATCGGCTGA22560.22356178718622322No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21850.21652593306821705No Hit
CATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATTTTG21680.2148412919413705No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21470.21276026466703069No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG21440.21246297505641074No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG21300.21107562354018414No Hit
GTCCCTAAGCTCCTCATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCACTGGAT20840.20651718284401113No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20020.19839126682039843No Hit
GTTAACATACTGGTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAACT19880.19700391530417186No Hit
GTCCCAGATCCAGTGCCGCTGAACCTTGATGAGACCCCACTTTCTAAATT19620.19442740534546538No Hit
GAGTTAACATACTGGTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGACGGTGAGAGTGAACT19620.19442740534546538No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19530.19353553651360544No Hit
ATCTGGGACAGAGTTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGATT19500.19323824690298547No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19100.18927438542805244No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18660.1849141378056261No Hit
ATGTTAACTCTTCTCCGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAA18570.18402226897376617No Hit
GGTATCAGCAGAAGCCAGGGAAAGTCCCTAAGCTCCTCATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCTCAT18490.18322949667877955No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17850.1768873183188867No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17490.17331984299144698No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17400.17242797415958705No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17060.16905869190589395No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16720.1656894096522009No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT16690.1653921200415809No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16400.16251832047225445No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16280.16132916202977454No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16220.1607345828085346No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16210.16063548627166127No Hit
CATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCTCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAGTTCA15750.15607704557548827No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC15640.1549869836698817No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15570.1542933079117684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC14950.1481493226256222No Hit
ACCTTGATGAGACCCCACTTTCTAAATTAGACGCCTTATAGATGAGGAGCTTAGGGACTTTCCCTGGCTTCTG14710.14577100574066237No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14580.14448275076130915No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14570.1443836542244358No Hit
GCTTAGGGACTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAACTATTA14470.14339268885570255No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13610.13487038668459653No Hit
CCCTGGTCCCTTGGCCGAACGTCGGAGAAGAGTTAACATACTGGTGGCAGTAATAAGTTGCAAAATCATCAGG13540.13417671092648326No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13500.13378032477898996No Hit
GTATCAGCAGAAGCCAGGGAAAGTCCCTAAGCTCCTCATCTATAAGGCGT13490.13368122824211662No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13490.13368122824211662No Hit
TCTCCACCCTGGTCCCTTGGCCGAACGTCGGAGAAGAGTTAACATACTGGTGGCAGTAATAAGTTGCAAAATCAT13310.13189749057839675No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12760.12644718105036382No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12490.12377157455478403No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12490.12377157455478403No Hit
GGACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12470.12357338148103739No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12460.12347428494416406No Hit
GGATCTGGGACAGAGTTCACTCTCACCGTCAGCAGCCTGCAGCCTGATGA12430.12317699533354406No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12180.12069958191171094No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12100.11990680961672431No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC11800.11693391351052455No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11800.11693391351052455No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11070.10969986631877175No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11060.10960076978189845No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11040.10940257670815179No Hit
ACTTATTACTGCCACCAGTATGTTAACTCTTCTCCGACGTTCGGCCAAGGGACCAGGGTGGAGATCAAACGAA10820.10722245289693862No Hit
GCACTGGATCTGGGACAGAGTTCACTCTCACCGTCAGCAGCCTGCAGCCT10790.10692516328631864No Hit
GCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCACCAGTATGTTAACTCTTCTCCGACGTTCGGCCAAGGGA10750.10652877713882532No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10710.10613239099133204No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10660.1056369083069654No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACCCTGTCTGCATCTGTCGGAGA10540.10444774986448549No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10440.10345678449575223No Hit
ATCCAGTGCCGCTGAACCTTGATGAGACCCCACTTTCTAAATTAGACGCC10360.10266401220076563No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10130.10038479185267912No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10120.1002856953158058No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG200.007191645452.05151743
GTGGTAT2800.041.6899451
TCGCTAA350.001097743541.10828467
GTATCAA17500.038.845221
TATCAAC18750.035.690292
GTCTATA501.9151228E-434.3328971
ATCAACG19600.034.1424943
CAACGCA19800.033.9709435
AACGCAG20200.033.2982526
TCAACGC20250.032.8770944
AGAGTAC22450.030.28474211
GTGTATA1501.8189894E-1229.7551771
CGCAGAG23050.029.3401748
CAGAGTA23200.029.30570810
ACGCAGA23000.029.24897
TGTTAGA8700.029.22768670
GCAGAGT24050.028.1271789
AGTACGG19850.027.15902513
GAGTACG20250.026.6225512
TCGTATA658.928494E-426.3981422
Produced by FastQC (version 0.11.5)