FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933862
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC32300.3458755147976896No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC26540.28419616602881365No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT25430.2723100415264782No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGA20810.222838063868109No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20110.21534230967744697No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19880.21287941901480092No Hit
GTAATAGAGGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA18930.20270660975604532No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17770.19028507424009117No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGACTGTCATCCCATG17430.18664427934748387No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC17420.18653719714476014No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17100.18311056665760037No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16810.18000518277861183No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16570.177435209913242No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16200.17347316841246352No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16160.17304483960156855No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16150.1729377573988448No Hit
GCCCTGAGGGCCGCTGATTATTCCTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCA15030.1609445506937856No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14880.15933831765292944No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14480.15505502954397973No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13960.1494867550023451No Hit
CCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT13840.1482017685696602No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13760.14734511094787023No Hit
CTCTATTACTGTGCGAGAAGCATAACTGGGGATCCAGGCTACTGGGGCCC13630.1459530423124616No Hit
ATAATAATCAGCCTCATCCCCAGACTGGAGTCCACTGATGGCCAGGGAGG13360.14306182283892052No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12500.13385275340467864No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12360.13235360256654624No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12140.1299977941066239No Hit
GCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGG12080.12935530089028144No Hit
GATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTA11870.12710657463308284No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11580.12400119075409428No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11220.12014623145603955No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11180.11971790264514456No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11030.11811166960428843No Hit
ACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGAC11000.11779042299611721No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC10960.11736209418522223No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10950.11725501198249849No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA10630.1138283814953387No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG10460.11200798404903509No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC10450.11190090184631134No Hit
GTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTC10420.1115796552381401No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA10420.1115796552381401No Hit
TCCCCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGAGC10300.1102946688054552No Hit
CTATTACTGTGCGAGAAGCATAACTGGGGATCCAGGCTACTGGGGCCCGGGAGCCCTGGTCAGCGTCTCCTCAGC10280.1100805044000077No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10280.1100805044000077No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10260.10986633999456022No Hit
ATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTCCAC10190.10911676457549403No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG10160.1087955179673228No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA10090.1080459425482566No Hit
CAGTAATAGAGGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGATTCTTCGACATGTCTA9940.10643970950740046No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9880.10579721629105798No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9880.10579721629105798No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9830.1052618052774393No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9780.10472639426382056No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9770.10461931206109681No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9630.10312016122296441No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9610.10290599681751693No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9600.10279891461479318No Hit
GGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCC9590.10269183241206944No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9510.10183517479027951No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA9370.10033602395214711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2400.044.455311
TGGTATC2600.041.0246852
GGTATCA5900.037.917051
GTATCAA13500.037.2213631
GTGTATA807.2435796E-834.4170151
TATCAAC15400.032.620382
TACTGAT8000.032.4235470
TTCTAGT800.003517536432.42353470
ATCAACG16000.031.182073
TCAACGC16100.030.9883924
CAACGCA16400.030.421535
AACGCAG16950.029.435986
ACGGATA500.00777461127.57052626
TGTCTAC5400.027.48717766
CGCAGAG18000.027.3511948
AGAGTAC17750.027.15599411
TCTACTG5900.026.57538268
GTCTACT5650.026.51149267
CAGAGTA18500.026.05507510
GCAGAGT19050.025.66439
Produced by FastQC (version 0.11.5)