FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822481
Sequences flagged as poor quality0
Sequence length21-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATCACTGTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGG24780.30128355548638813No Hit
GTGATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGTGAGAACTGGTTCTTGGACTTGTCTACGGA23600.2869367195108458No Hit
GTGATAATCAGCTTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG23510.28584246930932145No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGG23220.2823165519932983No Hit
GTCTCTGGTGGCTCCATCAGCAGTCCTAGTTACTACTGGACCTGGATTCG20530.2496106293032909No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20220.24584154527581792No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19700.23951921077812133No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGGCTGTCATCCCATG18350.22310545775525514No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16510.20073412030186716No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC16310.19830245318736847No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16280.19793770312019365No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16040.19501970258279522No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15950.19392545238127082No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15790.19198011868967185No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15120.18383403385610123No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14850.180551283251528No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC14700.17872753291565396No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14670.17836278284847917No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACC14640.17799803278130436No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14390.174958448888181No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT13810.1679066142561348No Hit
ATGCTGCACAGTGATAATCAGCTTCATCCTCGGACCGGAGCCCACTGATG13430.16328644673858728No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGGGTC13020.15830152915386494No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG12780.15538352861646654No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTCCTAGTTACTACTGGA12670.15404611170349225No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12400.150763361098919No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC12390.15064177774319407No Hit
GCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGG12380.15052019438746914No Hit
GTGTATCACTGTGCGAGAATGTATTACTATGGTTCGGGGAGTTATCACTG12330.14991227760884446No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12160.1478453605615206No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11690.14213094284244865No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11570.1406719425737494No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11460.13933452566077514No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT11260.13690285854627643No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC11220.1364165251233767No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11100.1349575248546775No Hit
CCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT11070.1345927747875027No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10820.13155319089437933No Hit
ATCCCATGCTGCACAGTGATAATCAGCTTCATCCTCGGACCGGAGCCCAC10660.12960785720278037No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10500.1276625235111814No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10430.1268114400211069No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG10350.1258387731753074No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA10120.12304235599363389No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC10070.12243443921500921No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC10070.12243443921500921No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9970.12121860565775988No Hit
ACCCTCAGGAGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG9820.11939485532188586No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9670.11757110498601182No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9660.11744952163028691No Hit
GGAGTGGATTGGGCGTATTTATTGGAGCGGCAGCACCAAGTACAACCCCTCCCTCAGGAGTCGAGTCACCATGTC9660.11744952163028691No Hit
CTCCTGAGGGTCGTTGATTATTCTTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACTTGCTGGTACCAGTATA9510.11562577129441289No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9490.11538260458296301No Hit
GTACAACCCCTCCCTCAGGAGTCGAGTCACCATGTCCGTAGACAAGTCCAAGAACCAGTTCTCACTGAAGCTGAC9460.1150178545157882No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9340.11355885424708899No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9210.11197827062266485No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9100.11064085370969055No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8950.10881710337381655No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA8930.10857393666236666No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAAGCTGATTATCACTGTGCAG8760.10650701961504278No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT8730.10614226954786797No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8680.10553435276924329No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8370.10176526874177032No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT8370.10176526874177032No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG8360.10164368538604539No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8300.10091418525169579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)