FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070632
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC43100.4025659610398344No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC35820.33456874070642384No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT34600.3231736021340666No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31250.29188367244767577No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26510.2476107570108123No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGA25870.24163297939908393No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25680.23985832667060203No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24990.23341353518295735No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC24390.22780936867196197No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGACTGTCATCCCATG24340.2273423547960457No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24000.22416666043981498No Hit
GTAATAGAGGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA23530.21977673000620193No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23020.21501318847185588No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22560.2107166608134261No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22080.20623332760462978No Hit
GCCCTGAGGGCCGCTGATTATTCCTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCA21770.20333784157394885No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21710.20277742492284928No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20740.19371735573007345No Hit
CCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT19340.18064096720441758No Hit
ATAATAATCAGCCTCATCCCCAGACTGGAGTCCACTGATGGCCAGGGAGG19060.1780256894992864No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18500.17279513408902406No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18070.16877881475614404No Hit
GATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTA17990.16803159255467798No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17770.16597673150064635No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17550.16392187044661471No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17450.16298784269478214No Hit
GCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGG17310.16168020384221657No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16590.1549552040290221No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16240.15168610689760814No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16180.1511256902465086No Hit
GTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTC15990.14935103751802672No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15910.14860381531656067No Hit
CTCTATTACTGTGCGAGAAGCATAACTGGGGATCCAGGCTACTGGGGCCC15820.1477631903399114No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15820.1477631903399114No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15820.1477631903399114No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15560.14533471818514673No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15510.14486770430923043No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15380.1436534682318481No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA15300.14290624603038204No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15220.14215902382891601No Hit
GGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCC14920.1393569405734183No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14720.13748888506975318No Hit
ATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTCCAC14710.13739548229456994No Hit
TCCCCAGACTGGAGTCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGAGC14370.13421978793833922No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14360.13412638516315598No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG14340.13393957961278943No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14160.13225832965949083No Hit
ACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGAC14070.13141770468284153No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGTCTGGGGATG13910.1299232602799094No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG13830.12917603807844338No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13760.1285222186521606No Hit
GTCTGGGGATGAGGCTGATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGAC13670.1276815936755113No Hit
CTGTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAG13360.12478610764483035No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCACAGAATTACCG13280.1240388854433643No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13220.12347846879226475No Hit
CTATTACTGTGCGAGAAGCATAACTGGGGATCCAGGCTACTGGGGCCCGGGAGCCCTGGTCAGCGTCTCCTCAGC12940.12086319108713357No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12880.12030277443603404No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC12830.11983576056011777No Hit
CAGTAATAGAGGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGAACTGATTCTTCGACATGTCTA12670.11834131615718567No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12620.11787430228126938No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA12600.11768749673090287No Hit
CTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCTGTGACCTGGTACCAGCAACTCCCAGGAACGGCCCC12540.11712708007980333No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGG12460.11637985787833728No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG12450.11628645510315402No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT12380.11563263567687122No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC12260.11451180237467215No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12060.11264374687100705No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11980.11189652466954098No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC11960.11170971911917447No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11900.11114930246807493No Hit
GTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGAC11880.1109624969177084No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11830.11049548304179213No Hit
GTGCCAGACTTGGAGCCAGAGAACCGGTCAGGGAGCCCTGAGGGCCGCTG11800.11021527471624236No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCT11790.11012187194105912No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGAC11720.10946805251477632No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT11700.1092812469644098No Hit
TGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCC11600.10834721921257724No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11600.10834721921257724No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG11410.10657256648409538No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11320.10573194150744607No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11250.10507812208116328No Hit
GTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC11180.10442430265488048No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC11150.10414409432933072No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11070.10339687212786465No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGATCCAATATCGGCGGTAATTCTGTGAC10940.10218263605048232No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10940.10218263605048232No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10870.10152881662419955No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT10760.10050138609718372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5700.045.9535941
GTGGTAT2150.044.884911
TGGTATC2300.043.4439432
GTATCAA15550.042.3334581
TATCAAC17450.037.7135122
CAACGCA18900.034.820155
AACGCAG19100.034.6359336
ATCAACG19150.034.54553
GTGTATA1201.8189894E-1234.4651951
TCAACGC19250.034.0080644
CGCAGAG20450.031.8558988
AGAGTAC20250.031.665811
ATTAGAG450.00465180230.6271483
TATCCTA450.00465180230.6271485
GCAGAGT21300.030.5903689
CAGAGTA21000.030.53487810
TATAAGA1950.030.0381622
ACGCAGA22600.028.9738127
TACTGAT13300.028.61864370
TGTATAA1353.110472E-1028.0748832
Produced by FastQC (version 0.11.5)