FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660574
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28500.4314429571857203No Hit
GTATTACTGTCAGCAGTATGGTTCCTCACCATACACTTTTGGCCAGGGGA23730.35923303066726814No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16500.24978276468646965No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGACTGAAGTCTGCCCCAGACCCACTGCCT16320.24705786179898087No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16090.24357604144274522No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15720.23797485217401837No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14950.22631832315531644No Hit
GTATGGTTCCTCACCATACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCAT14460.21890053196159703No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14290.21632701256785766No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13630.20633570198039888No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAATTACTTAGCCTGGTACC13220.20012897873667446No Hit
GTGTATTACTGTCAGCAGTATGGTTCCTCACCATACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAA12360.18710999827422817No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12350.18695861478047882No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12340.18680723128672944No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12220.1849906293617369No Hit
GTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCCCCTGGCCAAAAGTG11930.18060050804300504No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10930.16546215866806746No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG10840.1640997072243231No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10370.15698468301810242No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10350.1566819160306037No Hit
GAACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGACTGAAGTCTGCC9940.1504751927868793No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9830.14880997435563614No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9710.14699337243064364No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGACTGAAGTCTGCCCCAGACCCACTGCCT9560.144722620024403No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9290.14063526569316986No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8920.13503407642444298No Hit
CTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCA8860.13412577546194673No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8720.13200640654945547No Hit
GTCTGGGGCAGACTTCAGTCTCACCATCACCAGACTGGAGCCTGAAGATT8610.13034118811821233No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8550.12943288715571608No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8290.12549691631823232No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8130.12307478041824232No Hit
GTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATCTACCAGGGCCACTGGCATCCCAGA8060.1220150959619967No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8040.12171232897449794No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7930.12004711054325481No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7690.11641390669326979No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7610.11520283874327478No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7520.11384038729953042No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7480.1132348533245329No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT7350.11126686790579102No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7240.1096016494745479No Hit
GCCCCAGACCCACTGCCTCTGAACCTGTCTGGGATGCCAGTGGCCCTGGT7240.1096016494745479No Hit
GTTCCTCACCATACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGA7190.10884473200580103No Hit
GTGCATCTACCAGGGCCACTGGCATCCCAGACAGGTTCAGAGGCAGTGGG7160.10839058152455289No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7120.1077850475495554No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCCATGGTGCATCTACCAGGGCCACTGGCATCCCAGACAGGTT7100.10748228056205664No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7080.10717951357455788No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7080.10717951357455788No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCAGCTTGGTCC7030.10642259610581101No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGACAAATTGTGTTGACGCAGTC6980.10566567863706414No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6880.10415184369957037No Hit
GTTCAGAGGCAGTGGGTCTGGGGCAGACTTCAGTCTCACCATCACCAGAC6760.10233524177457787No Hit
CCATACACTTTTGGCCAGGGGACCAAGCTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG6630.10036725635583599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.039.1712461
TATCAAC13450.034.9350972
GGTATCA5500.034.934411
TCAACGC14450.032.517444
CAACGCA14750.032.321125
AACGCAG14900.031.9957396
ATCAACG14700.031.9644243
TATACTG652.4152503E-531.6593155
AGAGTAC15850.029.23237411
CGCAGAG16350.029.1692398
GCAGAGT16450.028.792069
CCCTATA605.6095765E-428.5813272
CAATACC605.6095765E-428.5813274
ACGCAGA18000.026.4893747
CAGAGTA17950.026.1948410
GAGTACG15300.025.12384812
AGTACGG15400.024.96070913
GTGTATA1101.2081255E-624.9531481
TGTTAGA5750.024.5694670
CCTATAC700.001379746524.498283
Produced by FastQC (version 0.11.5)