FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577969
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGTGAGAACTGGTTCTTGGACTTGTCTACGGA10480.18132460391474284No Hit
GATTATCACTGTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGG8630.14931596677330444No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8110.1403189444416569No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7980.13806968885874502No Hit
GTCTCTGGTGGCTCCATCAGCAGTCCTAGTTACTACTGGACCTGGATTCG7330.1268234109441856No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG6880.11903752623410598No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC6870.11886450657388199No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6650.11505807404895418No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC6610.11436599540805821No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6550.11332787744671427No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG6420.11107862186380238No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACC6090.10536897307641067No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG5880.10173556021170686No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG5870.10156254055148285No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT5850.10121650123103489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4100.042.782311
GTATCAA9600.040.842431
TATCAAC10900.035.337182
TCAACGC11750.032.4881864
GTGGTAT1500.032.1007161
CAACGCA12000.031.8113485
TGGTATC1550.031.0625212
AACGCAG12550.030.6912566
AGAGTAC12750.029.69605411
CTACGGA3500.029.47616669
CAGAGTA12800.029.04223310
ACGCAGA13750.027.5244267
GTATGGA500.00784917827.5148981
GTATAAA500.00784917827.5148981
CGCAGAG13800.027.4294478
GCAGAGT14400.026.5324129
TACGGGT1306.4555934E-926.47495715
AGTACGG13150.025.91347713
ATCAACG14950.025.764233
GAGTACG13400.025.68688412
Produced by FastQC (version 0.11.5)