FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921163
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10510.11409489959974511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18700.042.1460381
GGTATCA8050.037.1951451
TATCAAC21650.036.381542
CAACGCA24200.032.5479475
TCAACGC24600.032.158534
ATCAACG24700.032.0283363
AACGCAG24600.032.018716
AGAGTAC25850.029.82293311
ACGCAGA26350.029.7649297
CGCAGAG26450.029.6588368
CAGAGTA26100.029.5372710
GTGTATA1551.8189894E-1228.8651961
GTGGTAT2900.028.4823681
GCAGAGT27650.028.2548899
GTATTAA1103.749119E-828.1587071
TACTAGA500.00784930427.516526
TGGTATC3450.024.9243832
CCTATAC700.001357393824.568323
GTATAAG2550.024.2937851
TGTATAA1602.2791937E-923.6470072
Produced by FastQC (version 0.11.5)