FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867446
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10980.12657848442439068No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10280.11850881783995776No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9870.1137822988405042No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9140.10536678940245273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23500.041.4555471
GGTATCA10650.039.2954641
GTGGTAT2650.033.655781
TATCAAC28900.033.5734062
ATCAACG29200.033.4633033
TCAACGC29650.032.8397944
CAACGCA29700.032.5536355
AACGCAG30650.031.5446286
GTACGGT450.00476544630.4757424
CGCAGAG32650.029.6106328
AGAGTAC32350.029.567311
GCAGAGT35050.027.9743339
CAGAGTA34300.027.8863610
ACGCAGA35250.027.5238427
TGTATAA2300.025.341242
AATAGCG2051.8189894E-1224.24736862
AGTACGG30000.024.11253713
ACGCGTA1001.6244849E-524.0301227
TGTTAGA5900.023.94649370
GAGTACG30800.023.82016812
Produced by FastQC (version 0.11.5)