FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867446
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10880.12542567491232884No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10050.11585735596221552No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9290.10709600367054548No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9220.10628903701210218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24600.040.953691
GGTATCA10950.036.1784551
TATCAAC27950.035.912572
GTGGTAT3300.034.1512151
CAACGCA29400.034.025245
ATCAACG29550.033.8525283
TCAACGC29850.033.6266754
AACGCAG30100.033.3473856
CGACTAG959.298674E-932.49618529
AGAGTAC31100.032.0740511
CGCAGAG32350.031.2535068
CAGAGTA32750.030.77103210
GCAGAGT33000.030.6414389
ACGCAGA35250.028.5755047
TTATCCG1254.5165507E-927.38096424
GAGTACG29600.025.620812
AGTACGG29600.025.5053913
TGTATAA1901.8189894E-1225.1568012
TATCCGA1401.5039404E-824.4557725
AGTACTT10000.024.25435413
Produced by FastQC (version 0.11.5)