FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771468
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA48420.6276345875655245No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39470.5116219985793319No Hit
GTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATT35100.454976745633001No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34110.4421440681920702No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33190.43021875178231633No Hit
TCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT32090.4159602212923932No Hit
GAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT28920.3748697288805239No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28870.37422161385825464No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27300.3538708021590008No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26330.3412973707269777No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26270.3405196327002546No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25290.32781657826377764No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24740.3206873130188161No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24310.3151135238273007No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23190.3005957473284699No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTT21870.28348551074056216No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21380.27713398352232366No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21240.2753192614599698No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC20560.2665048971571083No Hit
TCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA19630.2544499577429006No Hit
CAGTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATTTAAATAGTTCCTAATGTTCTGA19430.2518574976538236No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19160.24835767653356974No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18520.2400618042485236No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17540.22735874981204665No Hit
GAATTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTAC17350.22489591272742354No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16490.21374833434439278No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16080.20843379116178506No Hit
GTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAA14700.19054581654715425No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14590.18911996349816196No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14450.1873052414358081No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14420.18691637242244658No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14100.18276843627992345No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13610.17641690906168497No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13110.16993575883899267No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTCGTAGAGGTA13040.16902839780781576No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12650.16397310063411574No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12400.16073252552276956No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12360.1602140335049542No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11740.1521774072288157No Hit
GTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATTTAAATAGTTCCTA11700.15165891521100033No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11650.1510108001887311No Hit
TTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGA11590.15023306216200802No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11580.15010343915755417No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11310.14660361803730032No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT11280.14621474902393877No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11210.14530738799276186No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC11030.14297417391259262No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT10960.1420668128814157No Hit
CTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG10940.141807566872508No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10900.1412890748546926No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10770.1396039757967926No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10750.13934472978788492No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10750.13934472978788492No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAACATTAGGAACTATTTAAATTGGTATCAGCAGAAA10610.13753000772553106No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10430.13519679364536183No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10430.13519679364536183No Hit
GTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGA10370.13441905561863873No Hit
ACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTG10360.1342894326141849No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAACATTAGGAA10310.13364131759191566No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10300.13351169458746184No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10270.1331228255741003No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10250.1328635795651926No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT10220.13247471055183105No Hit
GTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT9960.12910451243603105No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9940.12884526642712335No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9870.12793790539594643No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT9830.12741941337813104No Hit
CTGTAACTCTGTTGACAGTAGTAAGATGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAATTCTGTC9390.1217160011821618No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCA9220.11951241010644642No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9170.11886429508417717No Hit
GTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT9160.11873467207972332No Hit
GGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCACTTTGCAAACTGGA9130.1183458030663618No Hit
GTAGTAAGATGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA9100.11795693405300026No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC8870.11497560495056179No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8720.11303125988375408No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8710.11290163687930024No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8440.1094018157590464No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC8390.10875370073677715No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG8390.10875370073677715No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8390.10875370073677715No Hit
GTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACT8260.10706860167887715No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8240.10680935566996946No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8160.1057723716343387No Hit
GTTCAGTGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTA8120.10525387961652331No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8080.10473538759870792No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8080.10473538759870792No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG8060.10447614158980022No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA8000.10369840356307715No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT7970.1033095345497156No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7940.10292066553635407No Hit
CCCCTAAACTCCTCATCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCA7900.1024021735185387No Hit
GTGATAGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGTCACATCTGGCA7900.1024021735185387No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7800.10110594347400022No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT7760.10058745145618483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9550.041.7056581
GTGGTAT1500.040.8501551
CGATTGT901.3952458E-436.22667370
TATCAAC11200.035.2370072
TCGTATG1600.034.5481440
CCTATAC400.002771676534.0417941
TCAACGC12000.032.8878754
CTAGCGC1700.032.1184928
ACTAGCG1700.032.1017927
CAACGCA12300.032.085735
TGTTAGA11400.032.03200570
ATCAACG12450.031.6991583
TGACTGG13350.031.2607770
TAGCGCT1750.031.21299429
AACGCAG12750.030.9532956
TATACAC1800.030.2417243
ACGCAGA13100.030.1262977
CTTATAC1950.029.6774651
TTATACA1850.029.424382
AGAGTAC13300.029.41746711
Produced by FastQC (version 0.11.5)