FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065227
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT12280.1152805927750611No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAG200.00758590751.3538171
GTATCAA24950.041.30261
GGTATCA9650.039.0253561
TCGTATG2950.036.3798240
TATCAAC29250.035.5702132
TATACAC4050.035.492213
CTTATAC3900.034.2358781
CTCGTAT3100.033.45509739
ATCAACG31000.033.3414153
TCAACGC31550.032.868664
TATGCCG3400.032.68238443
CGTATGC3400.032.61713841
CAACGCA32150.032.255255
ACGAGAC3800.031.53452922
AACGCAG33000.031.4244336
ACTCGAC3950.031.24192827
CGAGACT4100.029.22986823
CGCAGAG36300.028.5676638
TCTCGTA3750.028.53884538
AGACTCG4450.028.48585125
Produced by FastQC (version 0.11.5)