FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065227
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33580.3152379727513478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA28450.041.9653321
TATCAAC31300.037.3725472
GGTATCA10800.036.0069161
TCAACGC33100.035.441574
ATCAACG33150.035.2852443
CAACGCA33600.034.914175
AACGCAG34650.033.9545826
ACGCAGA36650.032.106197
TGGTATC4150.032.0493662
CGCAGAG37250.031.233068
AGAGTAC36950.030.93553211
CAGAGTA37750.030.64149510
GTGGTAT5250.029.888451
GCAGAGT39800.029.3204279
AGTACGG24600.028.2958213
GAGTACG26400.026.23731212
GTACGGG27200.025.08928914
TACGGGG11300.024.45987115
CGTGACT700.001422333524.3724512
GTGTATA4400.024.0333271
Produced by FastQC (version 0.11.5)