FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285159
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT5930.20795415890783736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2100.054.52857270
TCGTATG1350.048.9869540
CTCGTAT1350.046.29166839
CCAATAC306.9738313E-445.065993
ACGAGAC1450.044.3745822
ACGCGTA1500.043.01634227
TCTCGTA1400.042.0524438
GACGCGT1650.039.05072826
TATACAC1750.038.627993
CGTAGTA1700.038.10328330
ATGCCGT1800.037.04794344
TATGCCG1800.036.9671743
GCGTAGT1750.036.9231929
GTATCAA19850.036.805111
CGTATGC1800.036.7933641
AATCTCG1650.035.55947536
CGCGTAG1850.034.90271428
ATCTCCG1850.034.71907810
GTAATCT1700.034.3414434
AGACGCG1900.033.88862625
Produced by FastQC (version 0.11.5)