FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285159
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12150.426078082753832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG407.9144775E-752.05561439
GCCTAAT758.9125024E-751.144370
AGCCTAA509.111209E-750.99775369
TCCGACT200.00792140850.78507613
TAACCCG354.8197937E-448.56928669
TTTAGAC451.813687E-646.15535738
GTTCGAG350.001174483740.5369554
ATCCGAG350.001223095540.20413647
GTATCAA23450.035.768141
AAGCCTA651.1548578E-535.25097368
TCGTGTA601.5231886E-533.85671613
GTTATGC400.002863390433.8092233
ATACCCC601.5379035E-533.809223
TATCAAC25800.032.4987872
AACGCAG25700.032.2305836
CGTGTAG751.9266517E-631.59960214
ACGCAGA26250.031.571887
TATACCC652.6586733E-531.2085172
TCAACGC26900.030.7927864
AGAGTAC27050.030.79021311
Produced by FastQC (version 0.11.5)