FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956060
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28710.3002949605673284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7500.038.3806881
GTATCAA37100.035.7463571
TGGTATC8400.033.8410342
GGTATCA16950.031.9434721
TATCAAC41900.031.796582
ACTTTAG1053.66848E-430.72951370
AACGCAG44550.029.6745686
ATCAACG44900.029.443253
CAACGCA44900.029.3669745
TCAACGC45800.028.9394514
GTATAAA953.397272E-728.857661
TGCGTAA500.0075417927.74287241
CAGAGTA45800.027.53449210
ACGCAGA48400.027.3155217
AGAGTAC46100.027.28097311
CGCAGAG49200.026.8798038
GCAGAGT49300.026.8308949
CAATCGT1250.001016991625.81279270
TATACCG851.5164135E-424.1755945
AGTACGG49100.022.40362513
Produced by FastQC (version 0.11.5)