FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386613
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAACTGGCCATTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAACTGTGGCTGCACCAT46470.33513316260557197No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42130.3038338743398482No Hit
GTTGTTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCGGGCTGCTGATGGTGAGAGTGAACT36070.26013025984899896No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA33760.24347096125595247No Hit
GTCCCAGACCCACTGCCACTGAAGCCGGCTGGGACACCGGTGGCCCTGGT28580.2061137462291209No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26270.18945444763607439No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCCGCAGTCTGAAGATT25690.1852715934438809No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25450.1835407572264215No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24670.1779155395196785No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAACAACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCT24420.17611258512649167No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGTTTAACGTCAGGGAGA24070.17358844897603007No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACAGTCTCCC23250.16767475856637723No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22970.16565544964600795No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22900.1651506224159156No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22110.15945328653344518No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTCTGGTCCCAGGGCCGAAAGTG21700.15649644132861873No Hit
GAGTTCACTCTCACCATCAGCAGCCCGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAACAACTGG21650.15613585044998135No Hit
CATCTATGGTGCATCCACCAGGGCCACCGGTGTCCCAGCCGGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA21460.15476560511115936No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21170.1526741780150626No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21150.15252994166360764No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20310.14647201490249984No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19420.14005349726275462No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19270.13897172462684254No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC19250.13882748827538757No Hit
CTACTGGGGTTGGATCCGCCAGCCCCCAGGGAAGGGCCTGGAGTATATTGGGTACATCTATTACACTGGCATCAC18880.13615911577347103No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18650.13450039773173914No Hit
GTGTATTACTGTGCCAGAGACCTCGAAACGGGGATTTTTGACTCCTGGGG17550.126567398401717No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17460.12591833482016973No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGC17280.1246202076570752No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17050.12296148961534328No Hit
CTCTAACAGTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17020.12274513508816086No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16780.12101429887070149No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAAGCCGGCTGGGACACCG16110.11618238109696072No Hit
GTCTCCCGCCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAA15680.11308129954067933No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15330.11055716339021776No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15260.11005233616012543No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACAGTCTCCCCTGTTGAAG14970.10796090906402868No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14940.10774455453684627No Hit
GTACCAGCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACCGGTGTCCCAGC14800.10673490007666162No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAACAGTGATGACGCAGTC14770.1065185455494792No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14420.1039944093990176No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14110.1017587459514659No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14090.10161450960001095No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14030.1011818005456461No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13900.10024426426118896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)