FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386613
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAACTGGCCATTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAACTGTGGCTGCACCAT45370.32720016327554985No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42670.3077282558291318No Hit
GTTGTTATACTGTTGACAGTAATAAACTGCAAAATCTTCAGACTGCGGGCTGCTGATGGTGAGAGTGAACT35150.25349538768207136No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA32050.2311387532065544No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27910.20128182845538012No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCCGCAGTCTGAAGATT26680.1924112928409008No Hit
GTCCCAGACCCACTGCCACTGAAGCCGGCTGGGACACCGGTGGCCCTGGT26020.18765149324288755No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23590.17012677654111133No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22670.1634919043741837No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22570.16277072261690897No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAACAACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCTCCCAGGCT22070.15916481383053527No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21960.15837151389753304No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACAGTCTCCC21910.1580109230188957No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21860.1576503321402583No Hit
GAGTTCACTCTCACCATCAGCAGCCCGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAACAGTATAACAACTGG21340.15390018700242966No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTCTGGTCCCAGGGCCGAAAGTG21230.15310688706942746No Hit
GTAATACACGGCCGTGTCTGCGGCAGTCACAGAGTTTAACGTCAGGGAGA20950.15108757814905818No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19880.14337093334621845No Hit
CATCTATGGTGCATCCACCAGGGCCACCGGTGTCCCAGCCGGCTTCAGTGGCAGTGGGTCTGGGACAGAGTTCA19050.1373851247608381No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC18820.13572640671910619No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18610.13421192502882923No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC17990.12974059813372585No Hit
CTACTGGGGTTGGATCCGCCAGCCCCCAGGGAAGGGCCTGGAGTATATTGGGTACATCTATTACACTGGCATCAC17830.12858670732208624No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC17290.12469232583280265No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17070.12310572596679822No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17040.12288937143961581No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16810.1212306533978839No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16590.11964405353187947No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAAGCCGGCTGGGACACCG16280.11740839008432778No Hit
GTGTATTACTGTGCCAGAGACCTCGAAACGGGGATTTTTGACTCCTGGGG15900.11466789940668377No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15560.11221588143194965No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15450.11142258149894743No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT15360.11077351791740017No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAACAGTGATGACGCAGTC14600.10529253656211214No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGC14480.10442711845338244No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14150.1020472186543758No Hit
GTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAACAACTTAGCCTGGTA14070.10147027324855602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22750.041.260291
GGTATCA9000.038.2039681
TATCAAC25850.036.17012
ATCAACG28650.032.3951843
TCAACGC28900.032.1149524
CAACGCA29400.031.5687775
CACCGAA9800.031.3743870
TAACGTG553.3275044E-431.24995
AACGCAG29950.031.2186016
TATTAGA1450.030.8188692
AGAGTAC30800.030.15775911
CGCAGAG32600.028.8039938
GCAGAGT32550.028.641039
CTCGTAT1405.535705E-528.0366870
ACGCAGA33350.027.9418897
TTAACGG1004.95982E-727.63744235
TCCGTCG950.007808992627.5448170
GTATAGA1005.169459E-727.5068571
TCGTCAA1207.4022653E-427.25788570
CAGAGTA34800.026.59249310
Produced by FastQC (version 0.11.5)