FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243697
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26300.041.5431481
TATCAAC28800.037.9140932
ATCAACG30450.035.63413
TCAACGC31500.034.5553054
CAACGCA31850.034.283395
GGTATCA13850.033.7378351
AACGCAG32900.033.2936066
ACGCAGA36000.030.4266597
AGAGTAC36500.029.54066711
TCGTATG1650.029.38870440
CAGAGTA37300.028.9070910
CGCAGAG37750.028.8353928
GCGATAT500.00791254927.47204220
GCAGAGT40350.026.8922449
CTAGTAC907.165063E-626.7067833
ACGAGAC2550.025.59082422
CTCGTAT1902.5465852E-1123.66903139
TCTCGTA2053.6379788E-1223.6035938
AGTACGG31100.023.51812713
GAGTACG31650.023.21793412
Produced by FastQC (version 0.11.5)