FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1243697
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20400.16402709019962258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27100.042.1055531
TATCAAC29050.039.140772
TCAACGC30650.036.9843064
ATCAACG30750.036.8655133
CAACGCA31400.036.2099885
AACGCAG32900.034.7700586
ACGCAGA34950.032.7358787
AGAGTAC35150.032.2793211
CAGAGTA35800.032.0749510
CGCAGAG36450.031.3048138
ACTAACG450.004791495430.4428182
GCAGAGT38100.029.868779
AGTACGG29350.028.14690413
GAGTACG29700.027.81520812
GGTATCA14500.027.1765481
GTACGGG33200.024.57214714
CGCTAGT700.001390446724.46789718
TACGGGG17400.024.23037115
GTGGTAT6550.024.064731
ACGGGGA8350.022.16455816
Produced by FastQC (version 0.11.5)