FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562342
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC200.007492161451.5124131
TATCGTA250.00904889649.10853669
CGTATGC1100.037.7794941
GTATCAA14450.036.837021
TCGTATG1151.8189894E-1236.0948140
TACGCTG400.002238303435.55623265
CTCGTAT1102.7284841E-1134.5877639
TATCAAC15850.033.7849122
GGTATCA7200.032.9107061
GTGGTAT3300.032.26031
TACACAT1600.032.1809435
CTTATAC1400.031.8886341
AATCTCG1207.6397555E-1131.63741536
CAACGCA16950.031.5923825
TCAACGC16950.031.5923824
ATCAACG17100.031.3152563
AACGCAG17350.031.2597226
TTATACA1550.031.0044352
TGGTATC3300.030.1655712
TATACAC1750.029.4225773
Produced by FastQC (version 0.11.5)