FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938646
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19670.2095571706479333No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18320.19517475171683468No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17880.19048714850966178No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17360.18494725381027566No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAATTGGAATAGTTAT16920.18025965060310276No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTTCAAAAGTGAAT16270.1733347822288701No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14780.1574608531863983No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14010.14925754757384574No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCT13960.14872486539121246No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13550.14435687149361953No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGACT13020.13871044035770674No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12680.13508820151580042No Hit
GTCTGAGCCCTGACGACACGGCCTTGTATTTCTGTACAAGAGGGTCCGGTGGGGGGGCCCTACGACATATTGACT12400.13210518129305404No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12250.13050713474515419No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12180.12976137968946758No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11960.12741757808588114No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11580.12336919349786821No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11560.12315612062481489No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGATGGATTTTC11550.12304958418828822No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11510.12262343844218161No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11450.12198421982302168No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11380.12123846476733506No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11270.12006656396554186No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10900.11612471581405556No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10780.11484627857573568No Hit
CTCCAGGCCCTTCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTT10620.11314169559130918No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGGGGCCCTGTT10360.11037174824161612No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10310.10983906605898283No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAGTTACTTATCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT10200.1086671652571896No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA10000.1065364365266565No Hit
GTGCAGCCACAGTTCGTTTAATGTCCAGTCGTGTCCCTTGGCCGAAAGTG9800.10440570779612335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)