FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938646
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20090.21403170098205285No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18150.19336363229588152No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18030.19208519505756164No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16320.1738674644115034No Hit
TCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTTCAAAAGTGAAT16280.17344131866539675No Hit
GTCCAGGGAAGGGCCTGGAGTGGGTCTCAGGTATTAATTGGAATAGTTAT15380.16385303937799767No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13360.14233267919961304No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12790.13626010231759364No Hit
GTCTGAGCCCTGACGACACGGCCTTGTATTTCTGTACAAGAGGGTCCGGTGGGGGGGCCCTACGACATATTGACT12450.13263786347568732No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGGAGCCTGAAGACT12140.12933523394336097No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCGGGGGAAAGAGCCACCCTCTCCT12020.12805679670504108No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11940.12720450521282783No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11900.12677835946672122No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11900.12677835946672122No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11590.12347572993439487No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11510.12262343844218161No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11290.12027963683859516No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11180.11910773603680196No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11060.11782929879848207No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10820.11527242432184232No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10690.11388745064699578No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGACTGAGATGGATTTTC10480.11165018547993599No Hit
CTCCAGGCCCTTCCCTGGACCTTGCCGGACCCAGTGCATGGCATGATCTT10390.11069135755119608No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAGTTACTTATCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT10340.11015867536856282No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT10110.1077083373284497No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10020.1067495093997098No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGGGGCCCTGTT9930.1057906814709699No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9810.10451224423265001No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9730.10365995274043677No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9590.10216844262906358No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9590.10216844262906358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12250.038.2582781
GGTATCA5250.037.414351
TATCAAC14400.032.7696952
ACGGTTA552.9978954E-431.80932860
TCAACGC14950.031.5607594
CCGTTAA450.00464818230.6316316
ATCAACG15500.030.4424843
CAACGCA15700.030.2724465
AACGCAG16050.030.2560446
AGAGTAC17250.027.97397211
TACGGGT1555.2750693E-1126.68627415
TGGTATC3900.026.4954322
TTCGGGG8150.026.36736570
GTGGTAT3950.026.1725941
CAGAGTA18550.026.01353510
CGCAGAG18600.025.9353088
GCAGAGT18950.025.4630789
ACGCAGA19250.025.2331877
TATATTG700.001347170624.6002795
TACCGCG1300.001442973624.30927870
Produced by FastQC (version 0.11.5)