FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534963
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG25790.48208941552967216No Hit
GATTATTACTGTGCAACATTGGACGACAGTCTGACTAGTTGGGTGTTCGG25440.475546906982352No Hit
GCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCA23760.44414286595521557No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23420.43778728622353325No Hit
GACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCAACTAGTCAGA18030.33703265459480375No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17560.32824700025983106No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17530.3276862138129179No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15780.2949736710763174No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14870.27796314885328516No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14690.2745984301718063No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTC13820.2583356232113249No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC13430.25104539940145393No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13120.2452506061166847No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT12420.23216558902204454No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT11900.22244529060888324No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11450.21403349390518595No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11410.2132857786426351No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11050.20655634127967729No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA10750.20094847681054578No Hit
ACATTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGG10540.1970229716821537No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10490.19608832760396513No Hit
CCCCTGAGGGCCGCTGATTAGTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGGTACCAGTATA10420.19477982589450113No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT9980.18655495800644156No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA9840.18393795458751355No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9670.18076016472167233No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC9430.17627387314636714No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9400.17571308669945399No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC9310.17403072735871453No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9130.17066600867723564No Hit
GTGCAACATTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACC9100.17010522223032246No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9020.16860979170522072No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8830.1650581442081041No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8740.16337578486736465No Hit
GGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG8690.16244114078917607No Hit
GATTAGTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGG8630.1613195678953498No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG8620.16113263907971206No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8520.15926335092333488No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8450.15795484921387087No Hit
ATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATG8410.15720713395132002No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8300.1551509169793051No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAA8170.15272084237601477No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8110.1515992694821885No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7790.14561754738178154No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT7660.14318747277849123No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7600.14206589988466492No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC7460.13944889646573688No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7440.13907503883446146No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7410.1385142523875483No Hit
GTCCAATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC7390.13814039475627285No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG7290.1362711065998957No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC7210.13477567607479396No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7180.1342148896278808No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7110.13290638791841677No Hit
GTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGGTACCA7060.13197174384022822No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAACATTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGAC7040.13159788620895277No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCAA6870.12842009634311158No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC6850.12804623871183615No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT6750.12617695055545897No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6650.12430766239908181No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT6650.12430766239908181No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6630.12393380476780637No Hit
ATGTATACTGGTACCAGCAATTCCCAGGAATGGCCCCCAAACTCCTCATC6560.12262530305834235No Hit
TTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGGCTGCC6550.12243837424270464No Hit
CTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC6460.12075601490196518No Hit
GACTAGTTGGGTGTTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT6450.12056908608632746No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG6250.11683050977357314No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC6200.11589586569538454No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT6140.11477429280155824No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC6030.11271807582954335No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA6000.1121572893826302No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAG6000.1121572893826302No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTGATGATTATGCCTTTCACTGGGTCCGTCAAGCTCCGGGGAAGGGT5950.11122264530444162No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5950.11122264530444162No Hit
AATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGG5920.11066185885752847No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG5850.10935335714806445No Hit
CTCCTCATCTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATT5830.10897949951678902No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC5820.1087925707011513No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG5760.10767099780732499No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC5750.10748406899168729No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC5630.10524092320403468No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG5570.10411935031020839No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG5530.10337163504765749No Hit
ACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCAACTAGTCAGAC5510.10299777741638205No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC5490.10262391978510663No Hit
GTCGTCCAATGTTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCC5380.10056770281309174No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG5360.10019384518181632No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG5360.10019384518181632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATGT200.007301255651.84943436
TCGCAGT503.9244542E-450.64020570
TGGTATC1400.044.272062
GTATCAA8550.043.926831
GTGGTAT1500.043.6447371
TATCAAC9400.040.2949072
GGTATCA3200.039.8401761
AGGGGGG1006.9292855E-637.98015670
TCGTATG557.210858E-637.7689240
TCAACGC10400.036.4203954
ATCAACG10500.036.073533
CAACGCA10600.035.733225
GCCTAAG400.002611251834.4563671
AACGCAG11150.034.2794156
AGAGTAC11500.032.6372811
GAGTACT2900.032.05908212
CTCGTAT652.2728633E-531.94030839
CAGAGTA11800.031.80751610
CTTATAC652.3393643E-531.805881
TATACAC652.3498562E-531.785073
Produced by FastQC (version 0.11.5)