FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657103
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11780.17927174278613855No Hit
GTAATAGACAGCCGTGTCCTCGGCGCTCAGACTGTTCATTTGCAGATACA6880.10470200257798246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12150.041.4727821
TATCAAC14050.035.853452
GTGGTAT2600.035.596931
CAACGCA15100.034.2654845
ATCAACG15300.033.8201453
TCAACGC15350.033.7074174
AACGCAG15550.033.273886
CGTCTAA652.1236923E-532.2605550
AGAGTAC16450.031.26415411
CAGAGTA16900.030.63455610
CGCAGAG17600.029.6041838
ACGCAGA17900.029.0991637
GCAGAGT18200.028.8231459
AGTACGG15150.025.79963513
GTAATAG2000.025.7088931
TAATAGA2000.025.7010692
TACGGGG7700.025.3827415
GGTATCA7550.024.9711111
GAGTACG15900.024.58266812
AATAGAC2100.024.477213
Produced by FastQC (version 0.11.5)