FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961829
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18130.188495044337403No Hit
GTATTACTGTGCGAGAGTGGGCTATGGCAGCGCCTGGGGAAAGTTTGACTACTGGGGCCAGGGAACCCTGGTCAC15530.16146321227577873No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14310.14877904492378582No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14180.14742745332070462No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14060.1461798303024758No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG13960.1451401444539518No Hit
CAGTAATATGTAGCAATATCTTCAGGCTGCAGGTTGCTGATGGTGAAAGCAAAATCAGTCCCAGACCCACTTCCA13440.13973377804162693No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12990.1350551917232689No Hit
CAGTAATACAGTGCCGTGTCTTCAGATCTCAGGCTGCTCAGCTCCATGTA12580.13079247974432046No Hit
CCTGATATACGATGCATCCCATTTGGAAACAGGGGTCCCATCAAGGTTCA12280.12767342219874842No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12060.1253861133319956No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12050.1252821447471432No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12000.12476230182288119No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11700.12164324427730917No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG10580.10999876277384024No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGGGA10490.10906304551016865No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10440.10854320258590665No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10220.10625589371915382No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10030.10428049060695821No Hit
GTAGGAGACAGAGTCACCATCACTTGTCAGGCGAGTCAGGACATTAGGGA9930.1032408047584342No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9680.10064159013712416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.036.7202571
TATCAAC18450.031.7215062
GTGGTAT2850.031.4301151
CAACGCA19550.030.1127645
AACGCAG20100.029.4600626
TGTTAGA3900.026.93925570
AGAGTAC22000.026.60142711
TAATCGT1057.4968193E-726.35903538
ACGCAGA22350.026.3388777
CAGAGTA22400.025.97271510
CGCAGAG22700.025.932778
TCAACGC22950.025.6516134
ATCAACG23250.025.3206233
TCTATAC700.001350212724.5908323
GCAGAGT23850.024.5380049
AATAGCG1151.4350098E-624.46703762
TTAATCG1151.6633039E-624.05563237
CTTATAC953.093954E-421.759311
TATAAGA3350.021.5812072
GGTATCA15700.021.505221
Produced by FastQC (version 0.11.5)