FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869656
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT12250.14086029418528706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6000.042.86531
GTATCAA33750.037.1880261
CGTAAAT700.00213448535.9011170
TGGTATC7400.034.735672
CTCGTAT2350.033.87441339
TATCAAC38450.032.5343862
TATACAC2750.032.403113
GGTATCA16000.032.1489751
TCGTATG2600.031.98930540
ATCAACG39850.031.3913963
CTTATAC2650.031.0571231
CAACGCA40300.030.9558285
TCAACGC40400.030.8792044
TTATACA2800.030.6004682
AACGCAG41200.030.3627936
ACGCAGA43550.028.8030857
TATCTCG2900.028.5704436
AGAGTAC43600.028.37865411
CAGAGTA43800.028.2490710
CGTATGC2950.028.2251441
Produced by FastQC (version 0.11.5)