FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200010
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT9100.45497725113744314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1750.058.82026770
TGAATCG200.0075730951.3601534
TATACAC2200.042.9246333
CGTATGC1800.042.5839741
TTATACA2250.041.9707572
CTTATAC2150.040.8161471
TCGTATG1800.040.54361740
ATTCGCA458.767836E-539.1883847
TCTCGTA1700.038.65093638
TATGCCG2000.038.5345643
TACACAT2400.037.9423145
CTCGTAT1750.037.63344639
ACGAGAC2250.037.511422
AATCTCG1950.037.02430736
GCCGTCT2000.036.95572746
CCCACGA2300.036.6775819
CATGAAT2350.036.2874932
AGACGCT2350.035.97819525
CCGTCTT2100.035.26954347
GAGACGC2400.035.21124
Produced by FastQC (version 0.11.5)