FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482697
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT5530.11456462335585264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA1200.039.961022
GGTATCA6550.039.751371
GTATCAA14800.037.7316441
AGGGGGG1901.4551915E-1136.30912870
CTCGTAT1151.8189894E-1236.1421639
TATACAC1350.035.520913
CTTATAC1500.034.2594031
TATACTG400.002688166234.2523045
ACTAAGG1450.033.10548827
TATCAAC17350.032.17942
TAAGGCG1500.032.0285429
TCGTATG1359.094947E-1230.80712740
TTAACGG450.004644384630.63372835
TAATGGT450.00478579630.44649120
CGTATGC1600.030.3639641
GTGGTAT2500.030.1482751
GACTAAG1600.029.99562626
ATCAACG19100.029.9483493
AACGCAG18950.029.4624066
TCAACGC19500.029.3340244
Produced by FastQC (version 0.11.5)